NuPAGE™ WedgeWell™ Midi Bis-Tris Welcome Pack, 4 to 12%

The NuPAGE Bis-Tris Midi WedgeWell Welcome Pack provides all the necessary NuPAGE gels, buffers, and reagents needed to begin using the SureLock Tandem Midi Gel Tank.

Welcome Pack contents

• SureLock Tandem Midi Gel Tank (STM1001)
• NuPAGE Bis-Tris WedgeWell Midi Gels (2 boxes, 20 gels)
• NuPAGE MES SDS Running Buffer, 20X (NP0002)
• NuPAGE LDS Sample Buffer, 4X (NP0007)
• NuPAGE Sample Reducing Agent, 10X (NP0009)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa (26619)

About the SureLock Tandem Midi Gel Tank

The SureLock Tandem Midi Gel Tank enables rapid electrophoresis of midi gels using minimal buffer (∼520 mL/gel) in a leak-free system. With a setup time of ∼30 seconds, the tank efficiently runs midi gels while also providing consistent performance. The tank features two independent chambers, allowing electrophoresis of one or two gels at a time, which saves on buffer and limits waste. The tank is compatible with all Invitrogen pre-cast midi gels and midi gel cassettes.

About NuPAGE Bis-Tris Midi gels

NuPAGE Bis-Tris Midi protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine midi gels, NuPAGE Bis-Tris midi gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris Midi gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE midi gels for optimal results during day-to-day use.

Transfer and detection

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer (NP0006) for traditional wet transfer using the SureLock Tandem Midi Blot Module (STM2001). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 3 Gel Transfer Device (IB31001).

Convenient to use

NuPAGE Bis-Tris WedgeWell-format gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.