JC-1 染料(线粒体膜电位探针)
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JC-1 染料(线粒体膜电位探针)
Invitrogen™

JC-1 染料(线粒体膜电位探针)

JC-1 是一种新型阳离子羰花青染料,可在线粒体中积累。这种染料在低浓度下以单体形式存在并发出绿色荧光,类似于荧光素。在较高浓度下,这种染料形成 J-聚集体,其具有宽激发光谱,发射波长上限为 ∼590 nm。这些特性使得 JC-1 成为线粒体膜电位的灵敏标记物了解更多信息
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货号数量
T31685 mg
货号 T3168
价格(CNY)
6,648.00
飞享价
Ends: 31-Dec-2025
9,012.00
共减 2,364.00 (26%)
5 mg
添加至购物车
数量:
5 mg
价格(CNY)
6,648.00
飞享价
Ends: 31-Dec-2025
9,012.00
共减 2,364.00 (26%)
5 mg
添加至购物车
JC-1 是一种新型阳离子羰花青染料,可在线粒体中积累。这种染料在低浓度下以单体形式存在并发出绿色荧光,类似于荧光素。在较高浓度下,这种染料形成 J-聚集体,其具有宽激发光谱,发射波长上限为 ∼590 nm。这些特性使得 JC-1 成为线粒体膜电位的灵敏标记物。另一种具有相似特性的染料是 JC-9 (D-22421)。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
数量5 mg
运输条件室温
子细胞定位线粒体
颜色绿色
适用于(设备)荧光显微镜, 流式细胞仪
产品类型染料
Unit Size5 mg
内容与储存
2–6°C避光、防潮储存。

常见问题解答 (FAQ)

当我使用膜电位指示剂时,看到神经元周围出现了较高的背景,如何降低背景干扰?

如果使用我们的FluoVolt 膜电势试剂盒(货号F10488),该试剂盒包含一种背景抑制剂,可改善这一问题。对于其他指标剂,可以考虑使用BackDrop 背景抑制剂(货号R37603、B10511和B10512)。

快反应膜电位探针和慢反应膜电位探针有什么区别?

在周围电场的作用下结构变化的分子可用作检测瞬时(毫秒级)电位变化的快反应探针。慢反应染料则会进入去极化细胞,结合蛋白或膜。增强去极化会造成额外的染料流入,增强荧光强度;过极化的特征则是荧光强度下降。快反应探针通常用于完整心脏组织的电位活动成像,或测量药理刺激引起的膜电位变化。慢反应探针常用于探索线粒体功能和细胞活力。

你们提供哪些类型的膜电位指示剂?我该如何根据自己的试验选择?

膜电位指示剂选择指南请见此处(https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html)。

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.