来自人血清的转铁蛋白,Alexa Fluor™ 555 偶联物
来自人血清的转铁蛋白,Alexa Fluor™ 555 偶联物
Invitrogen™

来自人血清的转铁蛋白,Alexa Fluor™ 555 偶联物

转铁蛋白是一种单体血清糖蛋白(∼80,000 道尔顿),其通过受体介导的内吞作用结合到脊椎动物细胞表面的特定受体上,并递送多达 2 个 Fe3+ 原子 — 标记的了解更多信息
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货号数量
T353525 mg
货号 T35352
价格(CNY)
8,876.00
5 mg
添加至购物车
数量:
5 mg
价格(CNY)
8,876.00
5 mg
添加至购物车
转铁蛋白是一种单体血清糖蛋白(∼80,000 道尔顿),其通过受体介导的内吞作用结合到脊椎动物细胞表面的特定受体上,并递送多达 2 个 Fe3+ 原子 — 标记的 LDL 复合物是研究该现象的有用工具。携带铁的转铁蛋白进入核内体后,酸性环境即可促使铁离子从转铁蛋白–受体复合物中解离。铁释放后,脱铁转铁蛋白被回收至细胞膜中,其从受体中释放出来以结合更多的铁。因此,荧光转铁蛋白偶联物可以与荧光 LDL 配合使用,以区分溶菌体定向途径和内涵体回收通路。

这些实验通常是通过向培养细胞中加入荧光标记的转铁蛋白并通过显微镜进行分析。我们提供生物素化的转铁蛋白偶联物及超过 10 种荧光形式。

转铁蛋白规格:

标记 (Ex/Em):Alexa Fluor™ 555 (555/565)

含量:15 mg 固体(含 5 mg 转铁蛋白偶联物)

标记的转铁蛋白的主要应用
标记的转铁蛋白的其中一些应用包括:
•使用 FRET 对转铁蛋白受体动力学进行成像
• 通过共聚焦激光扫描显微镜检查观察活细胞中的受体转运
• 研究内涵体酸化期间发生的事件
• 测量哺乳动物和寄生虫中的转铁蛋白受体结合亲和力

仅供研究使用。不得用于任何动物或人类的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型Alexa Fluor 染料
激发/发射555/565
形式实心
蛋白质家族转铁蛋白
数量5 mg
运输条件室温
产品线Alexa Fluor
产品类型转铁蛋白
pH7.2
Unit Size5 mg
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

引用和文献 (19)

引用和文献
Abstract
TrkA receptor activation by nerve growth factor induces shedding of the p75 neurotrophin receptor followed by endosomal gamma-secretase-mediated release of the p75 intracellular domain.
Authors:Urra S, Escudero CA, Ramos P, Lisbona F, Allende E, Covarrubias P, Parraguez JI, Zampieri N, Chao MV, Annaert W, Bronfman FC
Journal:J Biol Chem
PubMed ID:17215246
'Neurotrophins are trophic factors that regulate important neuronal functions. They bind two unrelated receptors, the Trk family of receptor-tyrosine kinases and the p75 neurotrophin receptor (p75). p75 was recently identified as a new substrate for gamma-secretase-mediated intramembrane proteolysis, generating a p75-derived intracellular domain (p75-ICD) with signaling capabilities. Using PC12 cells ... More
VAMP4 cycles from the cell surface to the trans-Golgi network via sorting and recycling endosomes.
Authors:Tran TH, Zeng Q, Hong W
Journal:J Cell Sci
PubMed ID:17327277
'VAMP4 is enriched in the trans-Golgi network (TGN) and functions in traffic from the early and recycling endosomes to the TGN, but its trafficking itinerary is unknown. Cells stably expressing TGN-enriched VAMP4 C-terminally-tagged with EGFP (VAMP4-EGFP) are able to internalize and transport EGFP antibody efficiently to the TGN, suggesting that ... More
Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy.
Authors:Prabhat P, Gan Z, Chao J, Ram S, Vaccaro C, Gibbons S, Ober RJ, Ward ES
Journal:Proc Natl Acad Sci U S A
PubMed ID:17384151
'The intracellular events on the recycling pathway that lead from sorting endosomes to exocytosis at the plasma membrane are central to cellular function. However, despite intensive study, these processes are poorly characterized in spatial and dynamic terms. The primary reason for this is that, to date, it has not been ... More
Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins.
Authors:Pellinen T, Arjonen A, Vuoriluoto K, Kallio K, Fransen JA, Ivaska J
Journal:J Cell Biol
PubMed ID:16754960
'Dynamic turnover of integrin cell adhesion molecules to and from the cell surface is central to cell migration. We report for the first time an association between integrins and Rab proteins, which are small GTPases involved in the traffic of endocytotic vesicles. Rab21 (and Rab5) associate with the cytoplasmic domains ... More
High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells.
Authors:Ram S, Prabhat P, Chao J, Ward ES, Ober RJ,
Journal:Biophys J
PubMed ID:18835896
'Single particle tracking in three dimensions in a live cell environment holds the promise of revealing important new biological insights. However, conventional microscopy-based imaging techniques are not well suited for fast three-dimensional (3D) tracking of single particles in cells. Previously we developed an imaging modality multifocal plane microscopy (MUM) to ... More