TNP-ATP(2'-(或-3')-O-(三硝基苯基)腺苷 5'-三磷酸三钠盐)
TNP-ATP(2'-(或-3')-<i>O</i>-(三硝基苯基)腺苷 5'-三磷酸三钠盐)
Invitrogen™

TNP-ATP(2'-(或-3')-O-(三硝基苯基)腺苷 5'-三磷酸三钠盐)

核苷酸类似物 TNP-ATP 的核糖基团经过修饰,在水中基本不发荧光。TNP 核苷酸经历向半醌结构的平衡转变,具有波长相对较长的光谱特性;这种形式只有结合到某些蛋白质的核苷酸结合位点后才会发出荧光了解更多信息
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货号数量
T76022 mL
货号 T7602
价格(CNY)
7,549.00
2 mL
添加至购物车
数量:
2 mL
价格(CNY)
7,549.00
2 mL
添加至购物车
核苷酸类似物 TNP-ATP 的核糖基团经过修饰,在水中基本不发荧光。TNP 核苷酸经历向半醌结构的平衡转变,具有波长相对较长的光谱特性;这种形式只有结合到某些蛋白质的核苷酸结合位点后才会发出荧光。
仅供科研使用。不可用于诊断程序。
规格
标签或染料TNP:
产品类型TNP-ATP
数量2 mL
运输条件湿冰
Unit Size2 mL
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

常见问题解答 (FAQ)

What is the concentration of TNP-ATP (2'-(or-3')-O-(Trinitrophenyl) Adenosine 5'-Triphosphate, Trisodium Salt) (Cat. No. T7602)?

The concentration is 5 mg/mL. This information can also be found on the product COA.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (250)

引用和文献
Abstract
Fluorescence resonance energy transfer mapping of the fourth of six nucleotide-binding sites of chloroplast coupling factor 1.
Authors:Shapiro AB, Gibson KD, Scheraga HA, McCarty RE
Journal:J Biol Chem
PubMed ID:1832671
Equilibrium dialysis measurements of adenine nucleotide binding to chloroplast coupling factor 1 suggest that the enzyme has six binding sites for ADP, adenylyl-beta,gamma-imidodiphosphate (AMP-PNP), and 2'(3')-O-2,4,6-trinitrophenyl-ATP (TNP-ATP). High affinity binding at all six sites requires the divalent cation, Mg2+. Three of the nucleotide-binding sites, sites 1, 2, and 3, have ... More
Site-site interaction on mitochondrial F1-ATPase. Functional symmetry of the high-affinity nucleotide binding sites.
Authors:Tiedge H, Schäfer G
Journal:Biol Chem Hoppe Seyler
PubMed ID:2876715
Interactions between the high affinity binding sites on mitochondrial F1 were analysed by combined use of the nucleotide analogues 3'-O-(1-naphthoyl)-ADP (N-ADP) and 2'-3'-O-(2,4,6-trinitrophenyl)-ADP (TNP-ADP). The binding behaviour of F1 with respect to these ligands was studied by measuring the fluorescence of F1 and of TNP-ADP and the fluorescence anisotropy of ... More
Mitochondrial ATP synthase. cDNA cloning, amino acid sequence, overexpression, and properties of the rat liver alpha subunit.
Authors:Lee JH, Garboczi DN, Thomas PJ, Pedersen PL
Journal:J Biol Chem
PubMed ID:2137825
'The predicted amino acid sequence of the alpha subunit of the rat liver mitochondrial ATP synthase has been obtained by sequencing a cDNA for the alpha subunit. Analysis of the sequence shows that it contains the A and B consensus sequences found in many nucleotide-binding proteins. Twelve amino acids of ... More
Elimination of the hydroxyl groups in the ribose ring of ATP reduces its ability to phosphorylate the sarcoplasmic reticulum Ca(2+)-ATPase.
Authors:Coan C, Amaral Júnior JA, Verjovski-Almeida S
Journal:J Biol Chem
PubMed ID:8463222
'2''-Deoxyadenosine 5''-triphosphate, 3''-deoxyadenosine 5''-triphosphate, and 3''-amino-3''-deoxyadenosine 5''-triphosphate were substituted for ATP in the Ca2+ pumping cycle of the sarcoplasmic reticulum Ca(2+)-ATPase. The rate of phosphorylation of the enzyme decreased by more than an order of magnitude when either of the hydroxyl groups was eliminated from the ribose ring. This resulted ... More
Structural organization of chloroplast coupling factor.
Authors:Snyder B, Hammes GG
Journal:Biochemistry
PubMed ID:2859887
'Fluorescence resonance energy transfer measurements have been used to construct spatial maps for the accessible sulfhydryl of the gamma subunit (dark site) and the essential tyrosine residue of the beta subunits relative to previously mapped sites on the H+-ATPase from chloroplasts. The extent of energy transfer was measured between a ... More