NuPAGE™ WedgeWell™ Tris-Acetate Welcome Pack, 3 to 8%

The NuPAGE Tris-Acetate WedgeWell Welcome Pack provides all the necessary NuPAGE Tris-Acetate gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

Welcome Pack contents

• Mini Gel Tank (A25977)
• NuPAGE Tris-Acetate WedgeWell mini gels (2 boxes, 20 gels)
• NuPAGE Tris-Acetate SDS Running Buffer, 20X (LA0041)
• NuPAGE LDS Sample Buffer, 4X (NP0007)
• NuPAGE Sample Reducing Agent, 10X (NP0009)
• HiMark Pre-stained Protein Standard (LC5699)

About the Mini Gel Tank

The Mini Gel Tank is compatible with all Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.

About NuPAGE Tris-Acetate gels

NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional Laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels.

For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer buffer (NP0006) for traditional wet transfer using the Mini Blot Module (B1000). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 3 Gel Transfer Device (IB31001).

Convenient to use

NuPAGE Tris-Acetate WedgeWell-format gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.