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引用和文献 (6)
Invitrogen™
pDisplay™ 哺乳动物表达载体
pDisplay™ 是一种哺乳动物表达载体，设计用于重组蛋白在哺乳动物细胞表面的靶向表达。展示的蛋白可用于分析其与已知或推定配体相互作用的能力。通过将目的基因克隆到载体上的血小板源生长因子受体 (PDGFR) 的独特 N 端分泌信号和 C 端跨膜锚定结构域的同一阅读框中，将目的蛋白靶向并锚定于细胞表面了解更多信息
| 货号 | 数量 |
|---|---|
| V66020 | 20 μg |
货号 V66020
价格（CNY)
11,561.00
20 µg
数量:
20 μg
价格（CNY)
11,561.00
20 µg
pDisplay™ 是一种哺乳动物表达载体，设计用于重组蛋白在哺乳动物细胞表面的靶向表达。展示的蛋白可用于分析其与已知或推定配体相互作用的能力。通过将目的基因克隆到载体上的血小板源生长因子受体 (PDGFR) 的独特 N 端分泌信号和 C 端跨膜锚定结构域的同一阅读框中，将目的蛋白靶向并锚定于细胞表面。与仅在原核细胞中操作的噬菌体展示载体相比，pDisplay™ 载体提供了能在哺乳动物细胞中加工表面展示的目的蛋白的优势。因此，从 pDisplay™ 中表达的真核来源的重组蛋白更类似于其天然形式。这可能有利于更准确的配体结合相互作用。除 N 端细胞表面靶向信号和 C 端跨膜锚定结构域外，pDisplay™ 载体包含以下主要特点：
•T7 启动子/启动位点，用于体外转录正义 RNA 和对插入片段进行测序
•含有新霉素抗性标记以便使用 Geneticin™ 在哺乳动物细胞中进行稳定筛选
•SV40 起始点以便在表达大 T 抗原的细胞系（例如 COS-1 和 COS-7）中进行复制和简单的载体挽救
•氨苄青霉素抗性基因用于在大肠杆菌中进行筛选
•T7 启动子/启动位点，用于体外转录正义 RNA 和对插入片段进行测序
•含有新霉素抗性标记以便使用 Geneticin™ 在哺乳动物细胞中进行稳定筛选
•SV40 起始点以便在表达大 T 抗原的细胞系（例如 COS-1 和 COS-7）中进行复制和简单的载体挽救
•氨苄青霉素抗性基因用于在大肠杆菌中进行筛选
仅供科研使用。不可用于诊断程序。
规格
输送类型转染
产品类型哺乳动物表达载体
数量20 μg
选择试剂（真核生物）Geneticin™ (G-418)
载体pDisplay
克隆方法限制性内切酶/MCS
产品线pDISPLAY
促进剂CMV
蛋白标记IgK 前导序列、PDGFR 跨膜结构域、c-Myc 抗原决定簇标签、HA 标签, PDGFR Transmembrane Domain, c-Myc Epitope Tag, HA Tag
Unit Size20 µg
内容与储存
20 µg pDisplay 超螺旋载体，以溶于 TE 缓冲液中的 0.5 µg/mL 溶液形式提供，pH 值为8.0。储存在 -20°C 下。妥善储存时，可保证稳定储存 6 个月。
常见问题解答 (FAQ)
我使用你们的pDisplay载体在细胞表面表达我们的蛋白。使用anti-HA抗体进行Western杂交成功检测到该蛋白的表达，但使用anti-myc抗体的免疫荧光检测未能发现其在膜表面的表达。你们能提供一些帮助么？
我能否使用FACS细胞分选或基于磁珠的方法来分离我转染了pDisplay载体的细胞？
我使用pDisplay载体来表达一条细胞膜表面的受体蛋白。使用胰酶处理细胞时是否需要考虑该蛋白所受的消化作用？
如需向哺乳动物细胞中转染pDisplay空载体，是否会形成一条靶向定位在细胞膜上的多肽？
我听说你们提供一种有助于蛋白分泌的载体，能够靶向定位至细胞膜。这是哪一种载体？
引用和文献 (6)
引用和文献
Abstract
Delta/notch-like epidermal growth factor (EGF)-related receptor, a novel EGF-like repeat-containing protein targeted to dendrites of developing and adult central nervous system neurons.
Journal:J Biol Chem
PubMed ID:11950833
'We have identified a novel epidermal growth factor (EGF)-like repeat-containing single-pass transmembrane protein that is specifically expressed in the developing and mature central nervous system. Sequence analysis revealed that the 10 EGF-like repeats in the extracellular domain are closely related to those of the developmentally important receptor Notch and its
Molecular mechanisms of pre-T cell receptor-induced survival.
Journal:J Biol Chem
PubMed ID:12114514
'En route to maturing as T cell receptor (TCR) alphabeta-expressing cells, the development of thymocytes is contingent on expression of a pre-TCR complex comprising a TCRbeta chain paired with a surrogate TCRalpha chain, pre-Talpha (pTalpha). The pre-TCR has been proposed to promote cell survival, proliferation, differentiation, and lineage commitment. However,
Myelin-associated glycoprotein interacts with the Nogo66 receptor to inhibit neurite outgrowth.
Journal:Neuron
PubMed ID:12160746
Myelin inhibitors of axonal regeneration, like Nogo and MAG, block regrowth after injury to the adult CNS. While a GPI-linked receptor for Nogo (NgR) has been identified, MAG's receptor is unknown. We show that MAG inhibits regeneration by interaction with NgR. Binding of and inhibition by MAG are lost if
DNA immunization of mice and macaques with plasmids encoding hepatitis C virus envelope E2protein expressed intracellularly and on the cell surface.
Journal:Vaccine
PubMed ID:10217599
We analyzed the humoral immune response elicited by hepatitis C virus (HCV) E2 protein expressed in vivo after injection of plasmid DNA into mice and rhesus macaques. Three plasmids were used for immunization: a plasmid containing the entire sequence of the E2 and p7 genes (pE2); a plasmid encoding a
Distinct kinetics for binding of the CD46 and SLAM receptors to overlapping sites in the measles virus hemagglutinin protein.
Journal:J Biol Chem
PubMed ID:12065582
Measles virus (MV) is a human pathogen using two distinct cell surface receptors for entry into host cells. We present here a comparative analysis for binding of the MV receptors CD46 and SLAM to the measles virus hemagglutinin protein (MVH, Edmonston strain). Soluble monomeric and dimeric MVH variants were prepared