Varioskan™ ALF Multimode Microplate Reader

Thermo Scientific™

Varioskan™ ALF Multimode Microplate Reader

The Thermo Scientific Varioskan ALF Multimode Microplate Reader is a versatile, entry level reader for most common absorbance, turbidimetric, fluorescence, and luminescence microplate assays.

货号	质保期
VA000010C	1-year Standard

货号 VA000010C

价格（CNY)

1-year Standard

The Thermo Scientific Varioskan ALF Multimode Microplate Reader is a versatile, entry level reader for most common absorbance, turbidimetric, fluorescence, and luminescence microplate assays. Its photometric optical design is based on a xenon flash lamp and monochromator, enabling continuous wavelength selection from UV through visible range up to 1000 nm. The broad wavelength range enables many common assays, including nucleic acid and protein quantification, bacterial growth curves, ELISA, and cell viability.

Enhance your fluorescence and luminescence measurements with the filter-based optics

With the Varioskan ALF reader, fluorescence and luminescence measurements are performed with filter-based optics for optimal specificity and sensitivity. The system comes with three pre-installed excitation and emission filter sets suitable for many common fluorometric applications like Quant-IT DNA quantitation assays and PrestoBlue and AlamarBlue cell viability assays. Additionally, five open filter positions are available for customized fluorometric and luminometric measurements. The filter slots are easy to access on the instrument's side with no tools required for filter exchange. Also, commonly used FRET and BRET assays are easy to set up and perform thanks to Varioskan ALF reader's unique filter-based optics design.

This package includes a Varioskan ALF Multimode Microplate Reader (Cat. No. VA000010C) and a Rapid Exchange two-year extended warranty.

Achieve consistently accurate results with automatic gain adjustment

The automatic gain adjustment feature selects the ideal fluorometric or luminometric reading range for your instrument based on signal intensity in the well, eliminating the need to manually adjust measurement parameters. The results are consistent no matter what signals are measured.

Additional features

Kinetic and endpoint measurements for a variety of applications
Spectral scanning for photometric measurements
Separate optimized measurement modes for absorbance and turbidimetric measurements
A self-diagnostics system that includes a set of initialization tests and adjustments, ensuring instrument readiness for operation
Compatible with 6- to 384-well microplates with and without lids
Reads μDrop and μDrop Duo plates for microvolume analysis of DNA, RNA, and proteins
Performs onboard shaking and incubation for temperature-critical assays
Operated through SkanIt software
- Unlimited installation on multiple computers, no annual fee required
- Includes an extensive cloud-based library of ready-made protocols
- Two editions available: Research Edition for life sciences and Drug Discovery Edition with features for compliance with 21 CFR Part 11
- Multiple user interface languages available
- Automation compatibility for integration with automation interface
Thermo Scientific verification plates provide comprehensive and traceable validation of the instrument optical performance. Ready-made SkanIt software protocols provide easy to read reports with defined measurement parameter limits

Varioskan ALF Multimode Microplate Reader with 2-year Extended Warranty (A66711), REX is a Varioskan ALF specific package SKU including the instrument (VA000010C), and an extended warranty to 2 years which includes a rapid exchange service (REX) should the instrument get broken during the warranty period.

仅供科研使用。不可用于诊断程序。

吸光范围200 nm 到 1000 nm

准确度（光度）±1.0% + 0.003 Abs (0 to 2 Abs), ±2.0% (2.0 to 2.5 Abs) at 450 nm (96-well plate)

带宽Photometry: 2.5 nm

深度（公制）61 cm

尺寸（长 x 宽 x 高）61 x 34 x 40 cm

动态范围Fluorometry and Luminometry: ≥6 Decades

发射波长范围210-720 nm

激发波长范围200-710 nm

过滤器Three factory installed fluorometric filter pairs: Coumarin family (Excitation 345/25 nm, Emission 450/40 nm); Fluorescein family (Excitation 485/15 nm, Emission 525/15 nm); Resorufin family (Excitation 555/15 nm, Emission 615/45 nm)

适用于（应用）Photometric, fluorometric and luminometric microplate assays

高度（公制）40 cm

包括SkanIt software, Research Edition

线性度0 to 2.5 Abs, 2.0% at 450 nm (96-well plate)

测量速度Photometry: ≤10 sec. (fast mode); Fluorometry and Luminometry: ≤20 sec. (96-well plate)

精确度SD <0.003 Abs, or CV <1.0% at 450 nm

产品类型Multimode Microplate Reader

数量Microplate Reader + 1 year warranty

灵敏度Photometry (DNA): <1 ng/μL (96-well half area plate); Turbidimetry (formazin): <5 turbidity units (FTUs) (96-well plate); Fluorometry (fluorescein): 0.35 fmol/well (black 384-well plate); Luminometry (ATP): <275 amol/well (white 96-well plate)

软件语言版本English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese

光谱扫描速度≤16 sec. (Fast mode)

电压100/115/220/230 V

质保期1-year Standard

重量（公制）24.5 kg

宽度（公制）34 cm

检测器类型光电倍增管

培养箱温度Ambient +2° to 45°C

界面PC Software (Thermo Scientific SkanIt Software)

光源氙闪灯

产品线Varioskan

读取方式Endpoint, Kinetic

振荡线性, 圆周式, Double Orbital

软件类型SkanIt 软件

Unit Size1 each

常见问题解答 (FAQ)

Can relative fluorescence units (RFUs) be compared between two different plate readers?

Relative fluorescence units (RFUs) cannot be compared between two different microplate readers. RFU values are arbitrary and dimensionless, meaning that the actual numbers don't have a specific physical meaning, but the relative values are used for comparison. The critical measurement is the ratio of signal to background.

Whether the signal is low or high is 100% dependent on the baseline, i.e., if the sample signal is 2 and the baseline is 1, then it is a low signal but if the sample signal is 2 and the baseline is 0.01, then it is a very high signal. Instruments that typically give sample signals in the tens of thousands RFUs also typically have baseline RFUs somewhere in the tens or hundreds. For example, the Varioskan Microplate Reader is designed such that the baseline is kept below 0.1 RFU.