WesternBreeze™ 阻滞剂/稀释液（A 部分和 B 部分）
WesternBreeze™ 阻滞剂/稀释液（A 部分和 B 部分）
Invitrogen™

WesternBreeze™ 阻滞剂/稀释液（A 部分和 B 部分）

WesternBreeze® 阻滞剂/稀释液（A 部分和 B 部分）是一种优化、易于使用的阻滞剂和一抗稀释剂系统，可以在硝酸纤维素 (NC) 和聚偏二氟乙烯了解更多信息
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货号数量
WB705080 mL
货号 WB7050
价格（CNY)
2,080.00
80 mL
添加至购物车
数量:
80 mL
价格（CNY)
2,080.00
80 mL
添加至购物车
WesternBreeze® 阻滞剂/稀释液（A 部分和 B 部分）是一种优化、易于使用的阻滞剂和一抗稀释剂系统，可以在硝酸纤维素 (NC) 和聚偏二氟乙烯 (PVDF) 膜上实现低背景/高信号 Western 印迹检测。有足够用于 20 次微型印迹的试剂。
仅供科研使用。不可用于诊断程序。
规格
缓冲液印迹缓冲液
数量80 mL
膜兼容性硝酸纤维素、PVDF
产品线WesternBreeze
产品类型封闭液和稀释液
Unit Size80 mL
内容与储存
80 ml 阻滞剂/稀释液（A 部分）；
80 mL 阻滞剂/稀释液（B 部分）。

在 +4°C 下储存。

常见问题解答 (FAQ)

WesternBreeze化学发光检测试剂盒中的封闭液/稀释液和抗体洗涤液是否可以作为独立产品购买？

可以，您可使用以下货号单独购买这些产品：

•货号WB7001（封闭液/稀释液A）
•货号WB7002（封闭液/稀释液B）
•货号WB7050（封闭液/稀释液A & B组合装）
•货号WB7003（抗体洗涤液）

WesternBreeze显色法检测试剂盒中的封闭液/稀释液和抗体洗涤液是否可以作为独立产品购买？

可以，您可使用以下货号单独购买这些产品：

•货号WB7003（抗体洗涤液）
•货号WB7001（封闭液/稀释液A）
•货号WB7002（封闭液/稀释液B）
•货号WB7050（封闭液/稀释液A & B组合装）

Why is the actual band size on a western blot different from the predicted size of the protein?

Western blotting is based on the separation of proteins by their size on a gel. However, migration of proteins through the gel matrix is also affected by other factors, which may cause the observed band size to be different from the predicted size.

Common causes are:
-Post-translational modification; for example phosphorylation and glycosylation increase the size of the protein
-Post-translation cleavage; many proteins are synthesized as precursor proteins, and then cleaved to give the active form
-Multimers, for example dimerization of a protein. This is usually prevented under reducing conditions, although strong interactions can result in the appearance of higher bands
-Splice variants; alternative splicing may result in different sized proteins being produced from the same gene
-Relative charge; the composition of amino acids (charged vs. non-charged)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Can I purchase the Blocker/Diluent and Antibody Wash solutions from the WesternBreeze Chemiluminescent Detection kits as standalone products?

Yes, you may purchase them as standalone products using the Cat. Nos. listed below:

- Cat. No. WB7001 (Blocker/Diluent A)
- Cat. No. WB7002 (Blocker/Diluent B)
- Cat. No. WB7050 (Combo pack containing Blocker/Diluents A & B)
- Cat. No. WB7003 (Antibody Wash)

Note: Cat. Nos. WB7001 and WB7002 may be ordered by clicking the Quick Order button located at the top right of any page on our website.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all WesternBreeze™ 阻滞剂/稀释液（A 部分和 B 部分） FAQs