X-Rhod-1，AM，细胞通透性 - 特殊包装

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca²⁺. They have uses in many calcium signaling investigations, including measuring Ca²⁺ in cells and tissues that have high levels of autofluorescence and also for detecting Ca²⁺ release generated by photoreceptors and photoactivatable chelators. Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells. The fluorescence signal from these cells is generally measured using fluorescence microscopy.

• 用于多种钙信号传导研究，包括细胞和组织中 Ca²⁺ 的测定
• 可通过直接向含培养细胞的培养皿中添加溶解的指示剂，将这些钙指示剂的 AM 酯形式上样至细胞中
• 通常使用荧光显微镜检测这些细胞的荧光信号
• 在结合 Ca²⁺ 之后荧光增加，波长稍有迁移
• 另外，基于 BAPTA 的指示剂可结合各种重金属阳离子（例如 Mn²⁺、Zn²⁺、Pb²⁺），亲和力远高于 Ca²⁺
• 由于存在这些离子而引起的钙测量的扰动可以使用重金属选择性螯合剂进行控制
• 标签（Ca²⁺–结合形式的 Ex/Em）：X-rhod-1 (580/602nm)
• 结合 Ca²⁺ 后荧光强度增加：>100 倍
• 在不含 Mg²⁺的缓冲液中，Ca²⁺ 的 K_d：约700nM

钙检测、细胞分析、细胞活率、增殖和功能、离子稳态及信号传导