Zymosan A S. cerevisiae BioParticles™,Texas Red 偶联物
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Zymosan A <i>S. cerevisiae </i>BioParticles&trade;,Texas Red 偶联物
Invitrogen™

Zymosan A S. cerevisiae BioParticles™,Texas Red 偶联物

Molecular Probes™ BioParticles™ 产品线包括一系列荧光标记的热或化学灭活的细菌和酵母,其大小、形状和天然抗原性均各异。这些荧光 BioParticles™ 产品一直被荧光显微镜、定量光谱测定法和流式细胞分析用来研究吞噬作用。我们提供的大肠杆菌了解更多信息
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货号数量
Z284310 mg
货号 Z2843
价格(CNY)
2,979.00
飞享价
Ends: 31-Dec-2025
3,933.00
共减 954.00 (24%)
10 mg
添加至购物车
数量:
10 mg
价格(CNY)
2,979.00
飞享价
Ends: 31-Dec-2025
3,933.00
共减 954.00 (24%)
10 mg
添加至购物车
Molecular Probes™ BioParticles™ 产品线包括一系列荧光标记的热或化学灭活的细菌和酵母,其大小、形状和天然抗原性均各异。这些荧光 BioParticles™ 产品一直被荧光显微镜、定量光谱测定法和流式细胞分析用来研究吞噬作用。

我们提供的大肠杆菌(K-12 菌株)、金黄色葡萄球菌(Wood 菌株,不含蛋白 A)和酵母聚糖(酿酒酵母)BioParticles™ 偶联物共价标记有多种不同的荧光基团(偶联后已特别注意去除游离染料)。与荧光素标记的 BioParticles™ 偶联物的荧光不同(这种荧光在酸性环境中会被部分淬灭),Alexa Fluor™、BODIPY™ FL、四甲基罗丹明和 Texas Red™ 染料偶联物的荧光在 pH 3 至 10 范围内均匀明亮。

BioParticles 规格:
• 标记(激发/发射波长):Texas Red™ (∼595/615 nm)
• 颗粒:酵母聚糖(酿酒酵母
提供调理剂


使用 BioParticles 产品
BioParticles™ 偶联物以冻干粉形式提供。每 mg 固体大约 3 x 108 大肠杆菌金黄色葡萄球菌颗粒,以及每 mg 固体大约 2 x 107 酵母聚糖颗粒。BioParticles™ 偶联物可在选择用于吞噬作用检测的缓冲液中复溶。结合到细胞表面的 BioParticles™ 偶联物(但未内吞)的荧光可以被溴化乙锭、台盼蓝或其他淬灭基团淬灭。除细胞应用之外,荧光 BioParticles™ 偶联物也可在流式细胞术校准参考品分选细菌及酵母突变株时使用。这些小颗粒也可能是光散射研究的有用参考,因为其大小和形状各不相同。

查找更多 BioParticles™ 产品
我们提供了一系列染料标记和未标记的大肠杆菌(K-12 菌株)、金黄色葡萄球菌(Wood 菌株,不含蛋白 A)和酵母聚糖(酿酒酵母)BioParticles™ 产品。查看 Molecular Probes™ 手册中用于追踪受体结合和吞噬作用的探针—第 16.1 节,了解这些产品及其应用。

关于 pH 敏感性内吞作用检测,请参阅 pHrodo™ BioParticles™ 偶联物

仅供研究使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型Texas Red™
形式冻干粉
数量10 mg
运输条件室温
种属酿酒酵母
适用于(设备)荧光显微镜
产品线BioParticles、Texas Red
产品类型生物颗粒偶联物
pH3 - 10
Unit Size10 mg
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

常见问题解答 (FAQ)

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.

What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (7)

引用和文献
Abstract
Kruppel-like factor 2 (KLF2) regulates proinflammatory activation of monocytes.
Authors:Das H,Kumar A,Lin Z,Patino WD,Hwang PM,Feinberg MW,Majumder PK,Jain MK
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:16617118
The mechanisms regulating activation of monocytes remain incompletely understood. Herein we provide evidence that Kruppel-like factor 2 (KLF2) inhibits proinflammatory activation of monocytes. In vitro, KLF2 expression in monocytes is reduced by cytokine activation or differentiation. Consistent with this observation, KLF2 expression in circulating monocytes is reduced in patients with ... More
Importance of MEK in neutrophil microbicidal responsiveness.
Authors:Downey GP, Butler JR, Tapper H, Fialkow L, Saltiel AR, Rubin BB, Grinstein S
Journal:J Immunol
PubMed ID:9552001
'Exposure of neutrophils to inflammatory stimuli such as the chemoattractant FMLP leads to activation of responses including cell motility, the oxidative burst, and secretion of proteolytic enzymes. A signaling cascade involving sequential activation of Raf-1, mitogen-activated protein kinase (MEK), and extracellular signal regulated kinase (ERK) is also rapidly activated after ... More
Myosin light chain phosphorylation does not increase during yeast phagocytosis by macrophages.
Authors:de Lanerolle P, Gorgas G, Li X, Schluns K
Journal:J Biol Chem
PubMed ID:8349578
'We have studied the role of myosin II light chain phosphorylation in yeast phagocytosis by J774 cells. J774 cells, which are mouse cells of monocyte/macrophage lineage, ingest opsonized yeast particles, and the rate of internalization is linear for 60 min at 37 degrees C. Immunoprecipitation of myosin II from cells ... More
Ca2+ and synaptotagmin VII-dependent delivery of lysosomal membrane to nascent phagosomes.
Authors:Czibener C, Sherer NM, Becker SM, Pypaert M, Hui E, Chapman ER, Mothes W, Andrews NW
Journal:J Cell Biol
PubMed ID:16982801
Synaptotagmin (Syt) VII is a ubiquitously expressed member of the Syt family of Ca2+ sensors. It is present on lysosomes in several cell types, where it regulates Ca2+-dependent exocytosis. Because [Ca2+]i and exocytosis have been associated with phagocytosis, we investigated the phagocytic ability of macrophages from Syt VII-/- mice. Syt ... More
Polyethylene glycol-modified GM-CSF expands CD11b(high)CD11c(high) but notCD11b(low)CD11c(high) murine dendritic cells in vivo: a comparative analysis with Flt3 ligand.
Authors:Daro E, Pulendran B, Brasel K, Teepe M, Pettit D, Lynch DH, Vremec D, Robb L, Shortman K, McKenna HJ, Maliszewski CR, Maraskovsky E
Journal:J Immunol
PubMed ID:10861034
Dendritic cells (DC) are potent APCs that can be characterized in the murine spleen as CD11b(high)CD11c(high) or CD11b(low)CD11c(high). Daily injection of mice of Flt3 ligand (FL) into mice transiently expands both subsets of DC in vivo, but the effect of administration of GM-CSF on the expansion of DC in vivo ... More