抗体标记用 SiteClick™ sDIBO 炔烃试剂盒
抗体标记用 SiteClick™ sDIBO 炔烃试剂盒
抗体标记用 SiteClick™ sDIBO 炔烃试剂盒
抗体标记用 SiteClick™ sDIBO 炔烃试剂盒
Invitrogen™

抗体标记用 SiteClick™ sDIBO 炔烃试剂盒

使用 SiteClick™ 化学方法，轻松将 Alexa Fluor™ pHrodo™ 和生物素 sDIBO 炔烃标签连接至叠氮基修饰的抗体。
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货号标签或染料标记规模
S10906Alexa Fluor™ 6471 mg
C20027Alexa Fluor™ 488100-250 µg
C20029Alexa Fluor™ 647100-250 µg
C20031伯胺100-250 µg
C20034pHrodo™ iFL 红色100-250 µg
S10902生物素1 mg
S10903pHrodo™ iFL 红色1 mg
S10904Alexa Fluor™ 4881 mg
S10907生物素5 mg
S10908pHrodo™ iFL 红色5 mg
S10909Alexa Fluor™ 4885 mg
S10911Alexa Fluor™ 6475 mg
S10914pHrodo™ 深红色100-250 µg
S10915pHrodo™ 深红色1 mg
C20028Alexa Fluor™ 555100-250 µg
C20030生物素100-250 µg
货号 S10906
价格（CNY)
4,142.00
Each
添加至购物车
标签或染料:
Alexa Fluor™ 647
标记规模:
1 mg
价格（CNY)
4,142.00
Each
添加至购物车

利用 SiteClick sDIBO 炔烃生成位点特异性标记抗体偶联物用于抗体标记。我们的 SiteClick sDIBO 抗体标记试剂盒经过优化，可与 SiteClick 叠氮基修饰试剂盒（S20026、S10900 或 S10901 单独销售）结合使用，并含有足量试剂，可使用 100 μg、1 mg 或 5 mg 叠氮基修饰的 IgG 抗体进行 SiteClick sDIBO 炔烃标记偶联反应。抗体标记用 SiteClick sDIBO 炔烃采用无铜点击化学将二苯并环辛炔 (sDIBO) 标记的 Alexa Fluor 染料、pHrodo 染料或生物素共价连接至叠氮基修饰的抗体。当与抗体连接时，标记远离其抗原结合结构域。

使用 SiteClick sDIBO 炔烃标记创建您自己的共价标记的抗体偶联物，用于配合叠氮基修饰抗体使用。SiteClick sDIBO 炔烃抗体标记试剂盒包括 sDIBO 炔烃和抗体浓缩仪，可实现 Alexa Fluor 和 pHrodo 染料、生物素或您选择的分子的无铜共价结合。配合 SiteClick 抗体叠氮基修饰试剂盒（作为货号S20026、S10900、S10901 单独提供）使用，标记发生在重链 N-连接聚糖上，远离抗原结合结构域，可实现单独标记反应与多个抗体种类和亚型之间出色的重现性。针对共价连接 100 µg、1 mg 或 5 mg 叠氮基修饰 IgG 抗体的偶联反应，对用于抗体标记的 SiteClick sDIBO 炔烃试剂盒进行了优化。

建议使用 SiteClick 抗体叠氮基修饰试剂盒（货号S20026、S10900、S10901）将叠氮化物部分连接至未标记 IgG 抗体的重链。其他含有叠氮部分的工程改造抗体也适用。

当与 SiteClick 抗体叠氮基修饰试剂盒结合使用时，用于抗体标记的 SiteClick sDIBO 炔烃试剂盒允许您选择尝试不同的抗体荧光标记，通过链霉素亲和素连接其他分子，或通过胺反应性部分连接您自己的分子，具体取决于您的检测。我们的 sDIBO 炔烃是 DIBO 环辛炔的改良版，所产生偶联物的黏性较低并且在生物样品中产生的信号背景较低。
仅供科研使用。不可用于诊断程序。
规格
化学反应性叠氮化物
激发/发射650/665
过滤器类型Cy5
流式细胞仪激光线路红色
套装内容包含 1 小瓶 SiteClick Alexa Fluor 647 sDIBO 炔烃，用于在 50 µL DMSO 和 1 个抗体浓缩仪中进行抗体标记
标记方法点击化学
标记规模1 mg
标记目标叠氮基修饰抗体
标签或染料Alexa Fluor™ 647
数量for 1 mg of Ab
反应一部分sDIBO 炔烃
运输条件湿冰
颜色远红外
产品线Alexa Fluor, SiteClick
Unit SizeEach
内容与储存
在冰箱（2°C 至 8°C）中避光储存。

常见问题解答 (FAQ)

How is SiteClick Alexa Fluor 647 sDIBO Alkyne different from Click-iT Alexa Fluor 647 sDIBO Alkyne?

Structurally, they are the same. The only difference is in the manner of packaging. SiteClick Alexa Fluor 647 sDIBO Alkyne is packaged for labeling 1 to 5 mg of antibody per the SiteClick labeling protocol with the actual amount being proprietary. Click-iT Alexa Fluor 647 sDIBO Alkyne is packaged as a 0.5 mg of reagent for use with any copper-free click reaction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the general protocol for using the sDIBO alkynes?

The protocol is very simple. Expose the azide-modified sample to the sDIBO alkyne (final concentration in the range of 1 to 5 µM is a reasonable starting range) in aqueous buffer of media for 1-2 hrs. Optimize the final working concentration and incubation time per the sample/application. The azide-modified target must have the azide accessible for reacting with the sDIBO, and the labeling solution should not include any sodium azide.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How are the sDIBO alkynes different from the original DIBO alkynes?

Both the original DIBO alkynes and the sDIBO alkynes utilize the same fluorescent dyes or biotin for detection. The sDIBO alkynes have improved solubility in aqueous solutions and less propensity to bind non-specifically compared to the original DIBO alkynes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm planning to label my antibody using SiteClick Alexa Fluor 647 sDIBO Alkyne (Cat. No. S10906). However, my antibody is in the following storage buffer: 0.01% sodium azide (preservative), PBS, 40% glycerol, and 0.05% BSA. Will the labeling work?

The labeling should work with the buffer that your antibody is in. The sodium azide and glycerol will be removed in the buffer exchange with the purification column. This step also concentrates the antibody. The spin column has a 50 kDa MW cut-off, so it will also concentrate the BSA. However, this shouldn't be a problem as our in-house testing has shown that the SiteClick assay can label antibodies in the presence of BSA.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.