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Invitrogen
NFATC2 Monoclonal Antibody (25A10.D6.D2)
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NFATC2 Antibody (MA1-025) in ICC/IF
Immunofluorescence analysis of NFATC2 Monoclonal Antibody (25A10.D6.D2) was performed using 70% confluent log phase HeLa and IMR32 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with NFATC2 Monoclonal Antibody (Product # MA1-025) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary An... View More
产品信息
MA1-025
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
25A10.D6.D2
抗原
Synthetic peptide corresponding to residues A(51) I S S P S G L A Y P D D V L D Y G L(69) of mouse NFATc2-A, B and C isoforms.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-025 detects NFATc2 (NFAT1) from mouse, rat and human tissues. This antibody does not cross react with NFAT2 (NFATc, NFATc1).
MA1-025 has been successfully used in Western blot, ChIP, immunocytochemistry, immunoprecipitation, immunofluorescence, immunohistochemistry (frozen & paraffin), and gel shift procedures. By Western blot, this antibody detects an ~140 kDa protein representing phosphorylated NFAT1 in resting immune cells, and an ~120 kDa protein in stimulated cells that represents fully-dephosphorylated NFAT1.
The MA1-025 immunogen is a synthetic peptide corresponding to residues A(51) I S S P S G L A Y P D D V L D Y G L(69) of mouse NFAT1-A, B and C isoforms. The sequence of this N-terminal peptide is ~70% homologous with human NFAT1.
Antibodies to this protein were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell-based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization, including the selection of the most appropriate fluorescent DyLight conjugated secondary antibody, may have to be performed for your high content assay.
MA1-025 can be used with blocking peptide PEP-062.
靶标信息
The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: IB-IXL; KIAA0611; NF-ATc2; NF-ATp; NFAT pre-existing s; NFAT pre-existing subunit; NFAT transcription complex, preexisting component; Nuclear factor of activated T-cells, cytoplasmic 2; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2; nuclear factor of activated T-cells, preexisting component; OTTHUMP00000031291; preexisting nuclear factor of activated T-cells 2; T cell transcription factor NFAT1; T-cell transcription factor NFAT1
基因别名: AI607462; NF-ATc2; NF-ATp; NFAT1; NFAT1-D; NFATC2; NFATP
UniProt ID: (Human) Q13469, (Mouse) Q60591
Entrez Gene ID: (Human) 4773, (Rat) 311658, (Mouse) 18019
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
molecular_function
sequence-specific DNA binding
transcription regulatory region DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
cytokine production
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
myotube cell development
cell migration
positive regulation of B cell proliferation
calcineurin-NFAT signaling cascade
Fc-epsilon receptor signaling pathway
response to drug
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
B cell receptor signaling pathway
positive regulation of myoblast fusion
biological_process
positive regulation of gene expression
transcription, DNA-templated
