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DYKDDDDK Tag Antibody (740001) in ICC/IF
For immunofluorescence analysis DYKDDDDK - BNIP3 transfected HEK293 cells were fixed and permeabilized for detection of DYKDDDDK-Tag protein using Anti-DYKDDDDK Recombinant Rabbit Superclonal™ Antibody (Product # 740001, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that... View More
Product Details
740001
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
C. elegans,
Human,
Tag
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
20H18L16, 20H1L23, 8H2L5, 8H8L17
Immunogen
Synthetic peptide (DYKDDDDK)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody consists of four clones viz. 20H18L16 and 20H1L23 from the N terminal and 8H2L5 and 8H8L17 from the C terminal.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a mixture of recombinant antibodies co-expressed from a library of heavy and light chains, providing the best of both worlds the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets the exact population of the heavy and light chains in a recombinant rabbit Superclonal™ antibody can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called 'Recombinant polyclonal antibody', this product is now rebranded as 'Recombinant Superclonal™ antibody'. The physical product and the performance remain unchanged.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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