Invitrogen

beta-3 Tubulin Monoclonal Antibody (2G10)

Name: beta-3 Tubulin Monoclonal Antibody (2G10)
Brand: Invitrogen
SKU: MA1-118

高级验证

15个已发表图片

41 篇参考文献

查看其他38个Beta III Tubulin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

beta-3 Tubulin Antibody in Immunocytochemistry (ICC/IF)

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beta-3 Tubulin Antibody (MA1-118) in ICC/IF

Immunofluorescence analysis of beta III tubulin (green) in the ectoderm derived from human ES cells. Embryoid bodies (EBs) were generated from the H9 embryonic stem cell line (WiCell Research Institute, WA09) using Gibco® KnockOut™ Serum Replacement. After four days in suspension culture, EBs were plated on Geltrex™-coated tissue culture-treated polystyrene and continuously cultured for 21 days. EB cultures... View More

beta-3 Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

beta-3 Tubulin Antibody in Immunohistochemistry (Frozen) (IHC (F))

beta-3 Tubulin Antibody in Western Blot (WB)

beta-3 Tubulin Antibody in Flow Cytometry (Flow)

beta-3 Tubulin Antibody in Immunoprecipitation (IP)

beta-3 Tubulin Antibody in Immunohistochemistry (IHC)

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Immunogen image

Immunogen Image beta-3 Tubulin Monoclonal Antibody (2G10)

产品信息

MA1-118

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看7篇已发表文章 7篇已发表文章

免疫组化 (IHC)

Assay-dependent

查看11篇已发表文章 11篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:200

查看20篇已发表文章 20篇已发表文章

流式细胞分析 (Flow)

1:50

免疫沉淀 (IP)

5 µg

产品规格

种属反应

Bovine, Guinea pig, Hamster, Human, Mouse, Pig, Rabbit, Rat

已发表种属

Amphibian, C. elegans, Cynomolgus monkey, Human, Mouse, Rabbit, Rat, Zebrafish

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

2G10

抗原

A synthetic peptide corresponding to amino acids 436-450 from rat neuronal specific beta-3 tubulin.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536829

产品详细信息

MA1-118 was successfully used to detect E18 Sparague Dawley primary cortical neurons.

MA1-118 can be used for immunofluorescence analysis of beta III tubulin in the ectoderm derived from human embryonic stem cells.

Western blot analysis of MA1-118 detects an ~50 kDa protein in neuronal-type cells. In bovine, a unknown band at ~32 kDa is also detected. MA1-118 shows specificity to beta-3 Tubulin and is non-reactive to lysates from non-neuronal cell types (e.g. HeLa cell lysate).

靶标信息

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: beta 3 tubulin; beta tubulin 3; betaIII-tubulin; class III beta-tubulin; MC1R; melanocortin receptor 1; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; TUBB; tubulin beta; Tubulin beta-3; Tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3 class III; tubulin, beta, 3; tubulin, beta, 4

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基因别名: 3200002H15Rik; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; I79_000194; M(beta)3; M(beta)6; mc1R; TUBB3; TUBB4