Invitrogen

Lamin A/C Monoclonal Antibody (mab636)

Name: Lamin A\/C Monoclonal Antibody (mab636)
Brand: Invitrogen
SKU: MA3-1000

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16个已发表图片

35 篇参考文献

查看其他32个Lamin A/C抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Lamin A/C Antibody in Immunocytochemistry (ICC/IF)

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Lamin A/C Antibody (MA3-1000) in ICC/IF

Immunofluorescent analysis of Lamin A/C (green) in untreated U2-OS cells (A) or HeLa cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 0.3% BSA for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C (Product # MA3-1000), at a dilution of 1:100 for at leas... View More

Lamin A/C Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

Lamin A/C Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Lamin A/C Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Lamin A/C Antibody in Immunohistochemistry (IHC)

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Immunogen image

Immunogen Image Lamin A/C Monoclonal Antibody (mab636)

产品信息

MA3-1000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:2,000

查看15篇已发表文章 15篇已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（冰冻） (IHC (F))

1:100

免疫细胞化学 (ICC/IF)

1:100

查看11篇已发表文章 11篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Bovine, Human, Pig

已发表种属

C. elegans, Dog, Human, Mouse, Pig

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

mab636

抗原

Porcine lamin preparation.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_325377

产品详细信息

MA3-1000 detects lamin A/C from human, bovine, porcine and canine samples.

MA3-1000 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects ~ 70 and 65 kDa proteins corresponding to lamin A and C, respectively, from HeLa cell extract. This antibody can also be used for immunofluorescence and immunohistochemistry for subcellular localization experiments. MA3-1000 has been widely used for detection of lamin A/C control in siRNA experiments.

The MA3-1000 antigen is porcine lamin preparation.

靶标信息

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 70 kDa lamin; Lamin A+C mutant; lamin a-c; lamin A/C-like 1; Lamin AC; mutant 453W; Mutant lamin A/C; Prelamin-A/C; progerin mutant; renal carcinoma antigen NY-REN-32; RP11-54H19.1; unnamed protein product

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基因别名: CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; PRO1

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UniProt ID: (Human) P02545

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Entrez Gene ID: (Bovine) 404144, (Pig) 100126859, (Human) 4000

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功能

structural molecule activity protein binding protein phosphatase 1 binding

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