Invitrogen

NUDT3 Polyclonal Antibody

Name: NUDT3 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-30435
Availability: InStock

高级验证

2个已发表图片

2 篇参考文献

查看其他3个NUDT3抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

NUDT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 5

NUDT3 Antibody (PA5-30435) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using NUDT3 (Product # PA5-30435) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

NUDT3 Antibody — Nudt3 down-regulation increases expression of mRNAs implicated in cell motility. (A) RNA levels of nine mRNAs implicated in cell migration tested in MCF-7 cells expressing nontargeting shRNA (ConKD, open bar), shRNA against Nudt3 (Nudt3KD, filled bar), or shRNA against Nudt4 (Nudt4KD, gray bar) are shown. (B) Western blot analysis of Nudt3 protein levels in ConKD or Nudt3KD MCF-7 cells ectopically expressing GFP, Flag-tagged wild-type Nudt3 (Nudt3), or mutant Nudt3 (Nudt3EE/QQ). The band for Flag-Nudt3EE/QQ runs slightly faster than that for Flag-Nudt3 likely due to differences in protein charge by the introduction of glutamic acid residues into the mutant protein. (C) A subset of the mRNAs implicated in cellular movement derived from the 144 mRNAs detected to increase greater than or equal to twofold by RNA-seq analysis in Nudt3KD are shown. (D) Transcript levels of the indicated mRNAs were determined by qRT-PCR in ConKD or Nudt3KD cells complemented with the indicated proteins. RNA levels in A and D are derived from at least three independent experiments and normalized to 18S rRNA and presented relative to the value in control cells set to one. Error bars represent ±SD. P-values are denoted by asterisks. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001 (Student's t-test). {KD}

产品信息

PA5-30435

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human NUDT3. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.14 mg/mL

14 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2547909

产品详细信息

Recommended positive controls: IMR32.

Predicted reactivity: Mouse (97%), Rat (96%), Dog (99%), Pig (99%), Chicken (95%), Rhesus Monkey (100%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

NUDT3 belongs to the MutT, or Nudix, protein family. Nudix proteins act as homeostatic checkpoints at important stages in nucleoside phosphate metabolic pathways, guarding against elevated levels of potentially dangerous intermediates, like 8-oxo-dGTP, which promotes AT-to-CG transversions (Safrany et al., 1998 ).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Ap6A hydrolase; Diadenosine hexaphosphate hydrolase; Diphosphoinositol polyphosphate phosphohydrolase 1; DIPP-1; Endopolyphosphatase; m7GpppN-mRNA hydrolase; m7GpppX diphosphatase; Nucleoside diphosphate-linked moiety X motif 3; Nudix motif 3

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基因别名: DIPP; DIPP1; NUDT3

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Entrez Gene ID: (Human) 11165

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NUDT3 Polyclonal Antibody

NUDT3 Antibody (PA5-30435) in IHC (P)

产品信息

PA5-30435

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

规格

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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