ER Alpha Recombinant Protein, Ligand Binding Domain, GST Tag Recombinant Protein

产品详细信息

This Estrogen Receptor (ER) Alpha Ligand Binding Domain (LBD) is a 62.80 kDa recombinant human protein (amino acids 282-595) expressed as a GST fusion protein in baculovirus-infected insect cells. It was expressed and purified in the absence of exogenous ligands. ER Alpha-LBD recombinant protein binds [³H]-estradiol with sub-nM affinity in hydroxylapatite assays and binds specifically to estrogen response element (ERE) sequences.

ER Alpha-LBD recombinant protein is:
• Flexible—ideal for ligand binding and coregulator displacement assays developed in-house or partnered with our LanthaScreen™ ER Beta Coactivator Assay or LanthaScreen™ ER Alpha Competitive Binding kits
• Ready to use—active, purified, and tagged with a GST
• Convenient—available in bulk sizes

ER Alpha-LBD recombinant protein is manufactured under the strictest quality parameters. This product is guaranteed for 6 months after purchase. Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

Used in:
LanthaScreen™ TR-FRET ER Alpha Coactivator Assay Kit
LanthaScreen™ TR-FRET ER Alpha Competitive Binding Kit

For bulk reagents, email: !Mad-DiscoveryServices@lifetech.com

Protein Form: Recombinant, Ligand Binding Domain

靶标信息

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.