Invitrogen

Human CDK4/cyclin D3, GST Tag Recombinant Protein

Name: Human CDK4\/cyclin D3, GST Tag Recombinant Protein
Brand: Invitrogen
SKU: A33515

说明书

技术支持

说明书

技术支持

说明书

技术支持

产品信息

A33515

产品规格

种属

Human

Expression System

Insect cells

氨基酸序列

CDK4: 1-303, Cyclin D3: 1-292

分子量

60.0-58.8 kDa

分类

Recombinant

类型

Protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

See Label

纯化类型

purified

保存液

50mM tris HCl, pH 7.5, with 25% glycerol, 0.1mM PMSF, 150mM NaCl, 0.1mM EDTA, 0.25mM DTT, 10mM glutathione

内含物

no preservative

保存条件

-80°C, Avoid Freeze/Thaw Cycles

运输条件

Dry ice

产品详细信息

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This CDK4/cyclin D3 recombinant human protein (CDK4: full length, cyclin D3: full length) was expressed in insect cells.

CDK4/cyclin D3 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Adapta™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

Protein Form: Full Length, Recombinant, Active

The gene accession number for the subunits CKD4 and Cyclin D3 are NP_000066.1 and NP_001751.1.

靶标信息

CDK4, also known as Cyclin-Dependent Kinase 4, is a member of the cyclin-dependent kinase (CDK) family. These kinases are key regulators of cell cycle progression. CDK4 is particularly involved in driving the transition from the G1 phase to the S phase of the cell cycle.

Cyclin D3 is a regulatory subunit that binds to CDK4 and activates its kinase activity. The binding of Cyclin D3 to CDK4 is essential for the proper functioning of CDK4, as cyclins regulate the activation and specificity of CDKs.

Together, the CDK4/Cyclin D3 complex plays a crucial role in promoting cell cycle progression, specifically by phosphorylating the retinoblastoma protein (Rb), which leads to the release of E2F transcription factors and the subsequent activation of genes required for DNA synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。