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产品信息
29-8012-65
应用
建议稀释比
已发表文章
产品规格
种属
Mouse
Expression System
E. coli
氨基酸序列
Mouse IL-1 beta, amino acids Val118-Ser269 (Accession # P10749)
分子量
17 kDa
分类
Recombinant
类型
Protein
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
4°C
运输条件
Wet ice
产品详细信息
Description: mouse IL-1 beta, also called lymphocyte activating factor (LAF), endogenous pyrogen (EP), leucocyte endogenous mediator (LEM), mononuclear cell factor (MCF), is a ~17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1 beta is mostly cell associated with 23% amino acid homology with IL-1 alpha. The immune regulatory role of IL-1 beta is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.
Applications Reported: Mouse IL-1 beta RSG ELISA Lyophilized Standard has been reported for use in the Mouse IL-1 beta ELISA RSG (Product # 88-7013).
Applications Tested: The intended use of the Mouse IL-1 beta RSG ELISA Lyophilized Standard is as a standard for Mouse IL-1 beta ELISA (Product # 88-7013-88). Typically the standard is run in a series of 7 doubling dilutions from the 'top' standard. Please refer to the Certificate of Analysis included with each ELISA set for instructions on the proper reconstitution and dilution of the lyophilized standard.
Note: These are single-use vials. Use one time within 4 hours of reconstitution and discard. Reconstitute and dilute as suggested in the Certificate of Analysis or recovery may not be accurate.
Source: E. coli expressed amino acids Val 118-Ser 269 of mouse IL-1 beta accession # P10749.
靶标信息
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta
基因别名: Il-1b; IL-1beta; Il1b
UniProt ID: (Mouse) P10749
Entrez Gene ID: (Mouse) 16176
