Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
产品信息
RMIL310
产品规格
种属
Mouse
Expression System
E. coli
分子量
15.1 kDa
分类
Recombinant
类型
Protein
纯度
>96% by SDS-PAGE and HPLC analysis
活性
ED50 < 0.1 ng/mL; determined by dose-dependent proliferation of mouse FDC-P1 cells.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
产品详细信息
RMIL310 contains 134 amino acid residues.
Purity: >96% pure by SDS-PAGE and HPLC analysis.
Activity: One unit of activity is <0.1 ng/mL, as determined by proliferation assay of mouse IL-3-dependent FDC-P1 cells, corresponding to a specific activity of >1x10^7 units/mg.
Use: Interleukin-3 exerts activity in the range of 0.1 to 10 ng/mL for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 µg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.
靶标信息
Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: BP/BPA; Colony stimulating factor multiple; Eo-CSF; Hematopoietic growth factor; IL-3; ILN; Interleukin; Interleukin 3 (colony stimulating factor; Interleukin-3; Interleukin3; Mast cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; Multilineage colony stimulating factor; multiple); Multipotential colony-stimulating factor; P cell stimulating factor; P-cell-stimulating factor; PCSA
基因别名: BPA; Csfmu; HCGF; Il-3; Il3; MCGF; PSF
UniProt ID: (Mouse) P01586
Entrez Gene ID: (Mouse) 16187
