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Gibco
PeproGMP® Human VEGF-165 Recombinant Protein, PeproTech®
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PeproGMP® Human VEGF-165 Protein (GMP100-20-50UG) in Bioactivity
Cell proliferation assay analysis of PeproGMP® Human VEGF-165 Recombinant Protein, PeproTech (Product # GMP100-20-50UG) compared to Human VEGF-165 Recombinant Protein, PeproTech (Product # 100-20-1MG), demonstrating comparable bioactivity across product grades.
产品信息
GMP100-20-50UG
应用
建议稀释比
已发表文章
产品规格
种属
Human
Expression System
E. coli
氨基酸序列
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
分子量
38.2 kDa
分类
Recombinant
类型
Protein
纯度
≥ 98% by SDS-PAGE gel and HPLC analyses.
内毒素浓度
≤0.1 EU/µg
活性
Determined by the proliferation of HUVECs.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
-20°C or -80°C if preferred
运输条件
Ambient
产品详细信息
PeproTech Recombinant Human VEGF-165 is a 38.2 kDa, disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.
PeproGMP VEGF-165 can be used with PeproGMP BMP-4 and PeproGMP SCF in CTS StemScale medium for pluripotent stem cell differentiation, specifically for mesoderm and hematopoetic progenitor cell induction.
Reconstitute using sterile water (i.e., WFI) to a concentration of 0.1-1.0 mg/ml.
Additional product information, specifications, handling and reconstitution information are provided on the Certificate of Analysis.
All PeproGMP® products are manufactured using animal-derived component free materials.
Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter <1043> Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.
靶标信息
The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
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生物信息学
蛋白别名: H-VEGF-165; L-VEGF; M-VEGF-165; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; VEGF165; VPF
基因别名: MVCD1; VEGF; VEGFA; VPF
UniProt ID: (Human) P15692
Entrez Gene ID: (Human) 7422
