MOPS SDS Running Buffer is recommended for separating medium- to large-sized proteins. MOPS-SDS Running Buffer is suitable for protein gel electrophoresis. Use of MOPS buffer allows proteins to run slower than when using MES buffer.
TAE (50X), TRIS + acetate + EDTA is used in electrophoresis of nucleic acids in agarose and polyacrylamide gel. It acts as a running buffer and as a gel preparation buffer. It is also useful for nucleic acid separations viz. DNA and RNA.
HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.
Used as a biological buffer substance in the pH range 7—9; pKa = 8.3 at 20°C; pKa = 7.82 at 37°C. Used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pKa of 8.
Used as a biological buffer substance in the pH range 7—9; pKa = 8.3 at 20°C; pKa = 7.82 at 37°C. Used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pKa of 8.
MES, is often used as a running buffer for resolving very small proteins on bis-tris gels, also used as a biological buffer in plant cell cultures. It is also been used in various protein characterization assays. This sterile solution suitable for cell culture and molecular biology applications.
It used in some biochemical techniques that is maintain the pH within a relatively narrow range. Tris-HCl is commonly used to make TBS buffers. Tris Buffered Saline has been used as washing buffer for electrodes.
TRIS, 1.0M buffer soln., pH 7.5 is used as buffers for stabilizing and storing nucleic acids and proteins. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand.
TRIS-buffered saline (TBS, 10X), with 0.5% Tween 20 is used as a wash buffer and for sample preparation in general immunoassay applications, Tween 20 reduces nonspecific binding and protein:protein interactions.TBS is often used for diluting secondary antibodies or streptavidin-AP conjugate.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo...
PBS with EDTA is also used to disengage attached and clumped cells. PBS-EDTA is used as a buffer in procedures generating dendritic cells from monocytes. PBS+EDTA to get better cell sorting. PBS/EDTA may also be used directly without addition of trypsine for very gentle cell dissociation.
Tris Buffered Saline (TBS) is a pH stabilizing solution used for western blot and ELISA procedures. It is used as a convenient prepared buffer for use in various applications. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio.
Tris is used in pH control in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo...
Potassium phosphate buffer is typically used as a component for a wide variety of media used in the culture of microorganisms, as a component in phosphate buffered saline (PBS). In addition to helping maintain pH, it supplies essential phosphate.