RIPA buffer is used to lyse cells and tissues. It is used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and and is particularly useful for disruption of nuclear membranes in the...
It is widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions. Triton X-100 is a commonly used detergent in laboratories. Triton X-100 is widely used to lyse cells to extract protein or organelles, or to permeabilize the membranes of living cells.
For lysis of human red blood cells and can be used to lyse reticulocytes, erythrocytes, and mature RBCs. RBC Lysis Buffer for human is used in the isolation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).