Search
Search
查看更多产品信息 Veriti™ 384-Well Thermal Cycler - FAQs (4388444)
19 个常见问题解答
如果测试失败：
1.应确认：
◦TVK校准是最新的。
◦探头无破裂或损坏。
2.在棉签上滴2-3滴矿物油。用棉签在每个探头的圆锥表面涂上薄薄一层油，改善热量传递。
3.重复 多探头温度验证试剂盒使用指南(https://tools.thermofisher.com/content/sfs/manuals/4460108A.pdf)里“运行96孔仪器测试之前”的步骤。
4.应确认：
◦探头牢固固定在合适的检测孔里。
◦电缆与端口牢固连接。
◦带状电缆平放在热熔保险器下方并且无折叠。
◦加热盖关闭并锁住。
5.重复 多探头温度验证试剂盒使用指南(https://tools.thermofisher.com/content/sfs/manuals/4460108A.pdf)里“运行测试”步骤。
请注意，如果检测再次失败，请发送邮件至instrumentservices@thermofisher.com联系Thermo Fisher Scientific。
如果使用了错误的塑料耗材，或者没有使用正确的托盘/固定器，可能会发生这种情况。请查看说明书中关于正确上样的指南部分。此外，您还可以从这里(https://www.thermofisher .com/us/en/home/lifescience/pcr/pcr-plastics.html)查看兼容的塑料耗材：
Veriti热循环仪 - 上样(手册第3-5页)
ProFlex PCR系统 -上样(手册第25页)
2720热循环仪 - 上样(手册第3-5页)
请参考-Veriti PCR仪专用的 实验方案指南（https://tools.thermofisher.com/content/sfs/manuals/cms_070449.pdf）。
如需设定一个恒定温度，请在程序中选择无限（∞）标识。
带有96孔（快速与标准）模块的Veriti热循环仪拥有创新的VeriFlex模块用于增强PCR功能。六个独立的Peltier模块提供最大的配置多样性和灵活性，并提供两个关键优势：
1.PCR条件优化：每一模块均可单独设置一个特定温度，非常适合用户在PCR条件优化过程中对温度进行精确控制。
2.运行更多实验条件：六个Peltier模块也可用于在同一反应中设定六个不同的退火温度。
Auto Delta特性能够帮助您在循环阶段中逐渐增高或降低每一循环某一步骤的温度。其中一个常见应用就是降落PCR（touchdown PCR）。
96孔Veriti 热循环仪最大模块变温速率是 3.9 °C/秒，96孔快速Veriti 热循环仪最大模块变温速率是 5.0°C/秒，384孔Veriti 热循环仪最大模块变温速率是 3.7°C/秒， 60孔Veriti 热循环仪最大模块变温速率是 3.3°C/秒。
孔板与塑料耗材均为特定模块类型所专用。请在下列兼容的反应管、孔板和封口膜列表中选择适当的模块类型。
•Veriti 60孔
•Veriti 96孔 0.1 mL
•Veriti 96孔 0.2 mL
•Veriti 384孔
Veriti热循环以有以下四种形式：
Veriti 96孔热循环仪
Veriti 384孔热循环仪
Veriti 60孔热循环仪
Veriti 96孔快速热循环仪
If the test fails:
1.Verify that the:
-Calibration of the TVK is up-to-date.
-Probe tips are not cracked or damaged.
2.Dispense 2 to 3 drops of mineral oil onto a cotton swab. Use the swab to apply a thin film of oil to the cone surface of each probe to improve heat transfer.
3.Repeat the steps in “Before You Run 96-Well Instrument Tests” from the Multiprobe Temperature Verification Kit User Guide (http://tools.thermofisher.com/content/sfs/manuals/4460108A.pdf).
4.Verify that the:
-Probes are seated securely in the appropriate test wells.
-Cables are connected securely to the ports.
-Ribbon cable lies flat under the thermal cutout and is not crimped.
-Heated cover is closed and locked down.
5.Repeat the steps in “Run the Test” from the Multiprobe Temperature Verification Kit User Guide (http://tools.thermofisher.com/content/sfs/manuals/4460108A.pdf).
Please note, if the test fails again, contact us at instrumentservices@thermofisher.com.
This can happen if the wrong plastics are used, or if the tubes are not being used with the correct tray/retainer set. Check the manual for directions on appropriate loading of the sample. Also, you can check for compatible plastics from here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html).
Veriti Thermal Cycler - Loading Samples (p. 3-5 of the manual)
ProFlex PCR System - Loading Samples (p. 25 of the manual)
2720 Thermal Cycler - Loading Samples (p. 3-5 of the manual)
Please refer to the guidelines in this protocol (https://tools.thermofisher.com/content/sfs/manuals/cms_070449.pdf) specific to the Veriti Thermal Cycler.
To add an infinite hold, select the infinity symbol for program.
The Veriti Thermal Cycler with a 96-well block (Fast and Standard) features innovative VeriFlex Blocks for enhanced PCR functionality. Six separate Peltier blocks provide maximal versatility and flexibility and offer two key benefits:
1. PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
2. Run more experiments: the six Peltier blocks can also be used to run up to six different annealing temperatures in the same run.
The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.
The 96-Well Block has a 3.9 degrees C/sec max block ramp rate, the 96-Well Fast Block has a 5.0 degress C/sec max block ramp rate, the 384- Well Block has a 3.7 degrees C/sec max block ramp rate, and the 60-Well Block has a 3.3 degrees C/sec max block ramp rate.
The plates and plastics will be specific to the block type. Choose the appropriate block type below for a list of the compatible tubes, plates, and films.
-Veriti 60-Well
-Veriti 96-Well 0.1 mL
-Veriti 96-Well 0.2 mL
-Veriti 384-Well
The Veriti Thermal Cycler is available in four formats:
Veriti 96-Well Thermal Cycler (a 0.2 mL or 0.1 mL)
Veriti 384-Well Thermal Cycler
Veriti 60-Well Thermal Cycler
Veriti 96-Well Fast Thermal Cycler
There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.
As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.
Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf
Find additional tips, troubleshooting help, and resources within ourPCR Thermal Cyclers Support Center.