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V5 Tag Antibody (740058MHRP) in WB
Western blot was performed using V5 Tag Recombinant Mouse Monoclonal Antibody (TCM5), HRP (Product # 740058MHRP, 1:2,500) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 6 µg (Lane 1), empty vector control, 6 µg (Lane 2), Myc-p65-V5, 6 µg (Lane 3), Myc-p65-V5, 3 µg (Lane 4), Myc-p65-V5, 1.5 µg (Lane 5), V5-H3-His, 12 µg (Lane 6), V5-H3-His, 6 µg (Lane 7), mCherry-GST, 6 µg (Lane 8),DYKDDDDK-p65-HA, 6 µg (Lane 9) and H3-GST 6 µg (Lane 10) were electrophoresed using NuPAGE™ 4-12% B... View More
产品信息
740058MHRP
产品规格
种属反应
Tag
宿主/亚型
Mouse
/ IgG2b, kappa
Expression System
ExpiCHO
分类
Recombinant Monoclonal
类型
Antibody
克隆号
TCM5
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4, with proprietary stabilizer
内含物
proprietary preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
This TCM5 monoclonal antibody recognizes V5 tag at N-terminus and C-terminus of proteins.
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
靶标信息
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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