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V5 Tag Antibody (740058MP647) in ICC/IF
Immunofluorescent analysis of V5 Tag was performed using 70% confluent V5 Tag-H3-6XHis transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with V5 tag Recombinant Mouse monoclonal antibody (TCM5), Alexa Fluor™ Plus 647 (Product # 740058MP647, 1:200) at 4 degree Celsius overnight. Panel a) shows representative images of cells that were stained for detection and localization ... View More
Product Details
740058MP647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG2b, kappa
Expression System
ExpiCHO
Class
Recombinant Monoclonal
Type
Antibody
Clone
TCM5
Conjugate
Excitation/Emission Max
658/675 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.8
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This TCM5 antibody has been tested by western blot, immunocytochemistry and flow cytometric analysis of HEK-293E cells transiently transfected with V5 constructs. This may be used for immunocytochemistry at 5 µg/mL and for flow cytometry at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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