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Invitrogen
CD141 Recombinant Mouse Monoclonal Antibody (JAA17), Alexa Fluor™ Plus 647
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FIGURE: 1 / 5
CD141 Antibody (740179MP647) in ICC/IF
Immunofluorecence analysis of CD141 was performed using CD141 Recombinant Mouse Monoclonal Antibody (JAA17), Alexa Fluor™ Plus 647 (Product # 740179MP647) on 70-80% confluent A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, and then blocked with 2% BSA for 1 hour at room temperature. The cells were stained with CD141 Recombinant Mouse Monoclonal Antibody (JAA17), Alexa Fluor™ Plus 647 (Product # 740179MP647, 1:100) in 0.1% BSA, incubated at 4 degree Celsius overnight. Panel a) shows representative image of cells t... View More
Product Details
740179MP647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
JAA17
Conjugate
Excitation/Emission Max
658/675 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.8
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This JAA17 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and A431 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Thrombomodulin (TM) is also known as Fetomodulin (FM), endothelial anticoagulant protein, or glycoprotein P112. Thrombomodulin contains six repeated domains homologous with epidermal growth factor (EGF) and an amino terminal domain homologous to lectin-like protein. Through its accelerated activation of protein C, synthesis of TM is one of several mechanisms important in maintaining thrombo-resistance and thus reducing clot formation on the surface of the endothelial cells. Immunohistochemical staining of formalin-fixed tissue for TM has been used for the study of a variety of vascular tumors, meningiomas and choriocarcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BDCA-3; CD141; CD141 antigen; DC141; Fetomodulin; sCD141; soluble CD141; Thrombomodulin; TM
Gene Aliases: AHUS6; BDCA3; CD141; THBD; THPH12; THRM; TM
UniProt ID: (Human) P07204
Entrez Gene ID: (Human) 7056
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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