Invitrogen

CD141 Recombinant Mouse Monoclonal Antibody (JAA17), Alexa Fluor™ Plus 647

Name: CD141 Recombinant Mouse Monoclonal Antibody (JAA17), Alexa Fluor\u2122 Plus 647
Brand: Invitrogen
SKU: 740179MP647

高级验证

查看其他43个Thrombomodulin (CD141)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)
高级验证 (1)

CD141 Antibody in Immunocytochemistry (ICC/IF)

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CD141 Antibody (740179MP647) in ICC/IF

Immunofluorecence analysis of CD141 was performed using CD141 Recombinant Mouse Monoclonal Antibody (JAA17), Alexa Fluor™ Plus 647 (Product # 740179MP647) on 70-80% confluent A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, and then blocked with 2% BSA for 1 hour at room temperature. The cells were stained with CD141 Recombinant Mouse Monoclonal Antibody (JAA17), Alexa Fluor™ Plus 647 (Product # 740179MP647, 1:100) in 0.1% BSA, incubated at 4 degree Celsius overnight. Panel a) shows representative image of cells t... View More

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CD141 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested, owing to their inherent genetic constitution. CD141 Recombinant Mouse Monoclonal Antibody (JAA17), Alexa Fluor™ Plus 647 (Product # 740179MP647) specifically detects CD141 expression in A-431 cells (pink histogram) while low expression of CD141 is seen in Jurkat cells (blue histogram). {RE}

产品信息

740179MP647

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

0.125 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JAA17

偶联物

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

激发/发射光谱

658/675 nm 查看光谱

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A/G

保存液

proprietary buffer, pH 6.8

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

AB_3699445

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Applications Tested: This JAA17 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and A431 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Thrombomodulin (TM) is also known as Fetomodulin (FM), endothelial anticoagulant protein, or glycoprotein P112. Thrombomodulin contains six repeated domains homologous with epidermal growth factor (EGF) and an amino terminal domain homologous to lectin-like protein. Through its accelerated activation of protein C, synthesis of TM is one of several mechanisms important in maintaining thrombo-resistance and thus reducing clot formation on the surface of the endothelial cells. Immunohistochemical staining of formalin-fixed tissue for TM has been used for the study of a variety of vascular tumors, meningiomas and choriocarcinomas.

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