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Invitrogen
ATP7A Monoclonal Antibody (L60/4)
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ATP7A Antibody (MA5-27720) in ICC/IF
Immunofluorescent analysis of ATP7A in mouse NIH 3T3 (mouse fibroblast cell line). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ATP7A monoclonal antibody (Product # MA5-27720) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Copper Transporting ATPase 1 Antibody, D) M... View More
产品信息
MA5-27720
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans,
Human
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
L60/4
抗原
Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
1 µg/mL of MA5-27720 was sufficient for detection of Copper-transporting ATPase1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.|Detects approximately 180kDa in rat brain membrane preparations.
This antibody was formerly sold as clone S60-4.
靶标信息
ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ATP 7A; ATPase, Cu++ transporting, alpha polypeptide; Copper pump 1; Copper-transporting ATPase 1; Cu++-transporting P-type ATPase; FLJ17790; MC 1; Menke; Menkes disease-associated protein; Menkes disease-associated protein homolog; Menkes protein; Menkes syndrome; OHS; OTTHUMP00000062077
基因别名: ATP7A; DSMAX; MC1; MK; MNK; SMAX3
UniProt ID: (Human) Q04656, (Mouse) Q64430, (Rat) P70705
Entrez Gene ID: (Human) 538, (Mouse) 11977, (Rat) 24941
copper-exporting ATPase activity
copper ion transmembrane transporter activity
copper ion binding
protein binding
ATP binding
superoxide dismutase copper chaperone activity
copper-dependent protein binding
nucleotide binding
hydrolase activity
cation-transporting ATPase activity
metal ion binding
molecular_function
primary active transporter
blood vessel development
in utero embryonic development
blood vessel remodeling
regulation of oxidative phosphorylation
tryptophan metabolic process
catecholamine metabolic process
copper ion transport
cellular copper ion homeostasis
mitochondrion organization
lactation
locomotory behavior
response to iron(III) ion
response to zinc ion
detoxification of copper ion
copper ion import
plasma membrane copper ion transport
peptidyl-lysine modification
removal of superoxide radicals
cerebellar Purkinje cell differentiation
pyramidal neuron development
central nervous system neuron development
extracellular matrix organization
collagen fibril organization
hair follicle morphogenesis
ion transmembrane transport
T-helper cell differentiation
epinephrine metabolic process
norepinephrine metabolic process
dopamine metabolic process
serotonin metabolic process
positive regulation of catalytic activity
negative regulation of catalytic activity
pigmentation
skin development
elastic fiber assembly
lung alveolus development
neuron projection morphogenesis
cartilage development
positive regulation of oxidoreductase activity
elastin biosynthetic process
copper ion export
release of cytochrome c from mitochondria
tyrosine metabolic process
transport
ion transport
cation transport
regulation of gene expression
metal ion transport
hindlimb morphogenesis
norepinephrine biosynthetic process
negative regulation of neuron apoptotic process
ATP metabolic process
response to copper ion
dendrite morphogenesis
