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Identifyn
ATRIP Monoclonal Antibody (4H6H4/G3), Alexa Fluor™ 647, Identifyn™
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图: 1 / 8
ATRIP Antibody (DC-000067) in ICC/IF
Identifyn Detailed protocols can be found below under Documents & Downloads. Widefield SIM: HeLa cells were grown on acid-etched 1.5H cover glass to ~50% confluency. DNA damage was induced via treatment with 2 µM Etoposide–spiked complete growth media for 24 hours, followed by -20°C methanol fixation and blocked with Thermo Fisher Fish Serum Blocker (Product # 37527X3). The primary antibody, Mouse anti-Human ATRIP Monoclonal - Identifyn SRM Alexa Fluor 647, IgG2b - (4H6H4/G3), was incubated for 1 hour at room temperature at a dilution of 1... View More
产品信息
DC-000067
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
4H6H4/G3
抗原
Immunogen against recombinant full-length protein, Uniprot ID Q8WXE1, Gene ID 84126.
偶联物
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
50 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 10% glycerol
内含物
0.02% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.
Recommended dilution for traditional microscopy:
Widefield - 1:250-1:1,000
Widefield SIM - 1:250-1:1,000
Confocal - 1:250-1:1,000
Recommended dilution for Super Resolution microscopy:
SIM (Structured Illumination Microscopy) 1:100-1:1,000
Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:100-1:1,000
Detailed protocols can be found below under Documents & Downloads.
靶标信息
ATRIP is an 86 kDa protein that is phosphorylated by ATR, regulates ATR expression, and is an essential component of the DNA damage checkpoint pathway. It localizes to the intranuclear foci after DNA damage or inhibition of replication.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATM and Rad3-related-interacting protein; ATR IP; ATR-interacting protein; DNase III; DRN3; FLJ12343; unnamed protein product
基因别名: AGS1; ATRIP
UniProt ID: (Human) Q8WXE1
Entrez Gene ID: (Human) 84126
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
