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Invitrogen
Aggrecan Monoclonal Antibody (BC-3)
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Aggrecan Antibody (MA3-16888) in ELISA
ELISA analysis of Aggrecan was performed by coating Amine-binding Maleic Anhydride Activated Plates (Product # 15110) overnight with 100 µL of 2 µg/mL Aggrecan Interglobular Domain recombinant protein (Product # RP-77527). Plates were blocked for 1 hour at room temperature with StartingBlock Blocking Buffer (Product # 37538) and incubated with 100 µL per well of an Aggrecan monoclonal antibody (Product # MA3-16888) in duplicate at 25, 12.5, 6.25, 3.125, 1.563, 0.78 µg/100 µL with serial dilutions of for 2 hours at room temperature. The pla... View More
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.jpg?time=20220826?time=20220826 "Aggrecan Antibody (MA3-16888) in IHC (F)")
产品信息
MA3-16888
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Dog,
Horse,
Cat,
Guinea pig,
Human,
Sheep,
Pig,
Rabbit,
Rat
已发表种属
Bacteria,
Bovine,
C. elegans,
Human,
Mouse,
Rabbit,
Rat,
Zebrafish
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
BC-3
抗原
Synthetic peptide corresponding to human aggrecan ARGSV neoepitope.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 1mg/mL BSA, 30% glycerol
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Purified recombinant Aggrecan Interglobular Domain (IGD) protein (Amino acids T331-G458 with C-term His-tag, expressed in E. coli), was used to validate this antibody. The recombinant protein contains cleavage sites for aggrecanases (E373-A374 in aggrecan) and matrix metalloproteinases (N341-F342 in aggrecan). The recombinant protein was cleaved in vitro using recombinant Aggrecanase. Degradation of IGD and accumulation of cleaved fragments was visualized via MEMCode reversible protein staining, followed by Western Blot using the BC-3 antibody for the specific detection of the aggrecan neoepitope. The antibody will also recognize full length IGD, as shown by ELISA, however with significantly lower affinity.
MA3-16888 has successfully been used in ELISA, immunohistochemistry (frozen and FFPE) and Western blot applications. This antibody may be used to immunostain formalin-fixed, paraffin-embedded sections, as well as alcohol-fixed frozen sections and un-fixed snap frozen sections. Samples must be deglycosylated using 0.01 Units Chondroitinase ABC, 0.01 Units Keratanase and 0.0001 Units Keratanase II per 10 µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition.
MA3-16888 detects aggrecan in human, bovine, feline, canine, guinea pig, equine, porcine, rabbit, rat, and sheep samples.
靶标信息
Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: aggrecan 1; aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); Aggrecan core protein; aggrecan epidermal growth factor-like domain-1; aggrecan interglobular domain; aggrecan, structural proteoglycan of cartilage; cartilage aggregating proteoglycan; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; high density cartilage proteoglycan; large aggregating proteoglycan; large aggregating proteoglycan, antigen
基因别名: ACAN; Agc; AGC1; AGCAN; CSPCP; CSPG1; CSPGCP; MSK16; SEDK
UniProt ID: (Bovine) P13608, (Human) Q9UCP4, (Rat) P07897
cell adhesion
skeletal system development
proteolysis
central nervous system development
keratan sulfate biosynthetic process
extracellular matrix disassembly
extracellular matrix organization
keratan sulfate catabolic process
cartilage condensation
ossification
chondrocyte development
response to radiation
response to mechanical stimulus
response to gravity
response to glucose
multicellular organism aging
response to acidic pH
response to organic cyclic compound
spinal cord development
proteoglycan biosynthetic process
collagen fibril organization
negative regulation of cell migration
response to drug
chondroblast differentiation
cellular response to growth factor stimulus
