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Rockland
BUB1 Polyclonal Antibody
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FIGURE: 1 / 3
BUB1 Antibody (600-401-Z98) in ICC/IF
Immunocytochemistry of Bub1 antibody. Tissue: A20 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Bub1 antibody at 10 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Bub1 is nuclear and occasionally cytoplasmic. Staining: Bub1 as a precipitated brown signal with hematoxylin purple counterstain.
Product Details
600-401-Z98
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Bub1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 19 amino acid synthetic peptide from near the N-terminus of human Bub1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The BUB1 gene encodes a serine/threonine-protein kinase that is integral to the spindle assembly checkpoint, a crucial mechanism ensuring proper chromosome alignment and segregation during mitosis. BUB1 is involved in detecting and correcting errors in kinetochore-microtubule attachments, which is vital for maintaining genomic stability. It acts by delaying the onset of anaphase until all chromosomes are correctly attached to the spindle apparatus. BUB1 is recruited to kinetochores in early mitosis, where it phosphorylates several substrates to activate other checkpoint proteins, such as BUBR1 and MAD2, thereby preventing premature progression through mitosis. Mutations or aberrations in the expression levels of BUB1 are often associated with aneuploidy and chromosomal instability, contributing to cancer progression. BUB1 is thus considered a potential target for cancer therapeutics, aiming to exploit its role in the mitotic checkpoint to selectively eliminate tumor cells through checkpoint bypass or mitotic arrest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1, mitotic checkpoint serine/threonine kinase; BUB1A; budding uninhibited by benzimidazoles 1 homolog; hBUB1; mBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase
Gene Aliases: AL022991; BUB1; BUB1A; BUB1L; C80208; D2Xrf87; hBUB1
UniProt ID: (Human) O43683, (Mouse) O08901
Entrez Gene ID: (Human) 699, (Mouse) 12235
protein phosphorylation
apoptotic process
sister chromatid cohesion
regulation of sister chromatid cohesion
mitotic nuclear division
mitotic cell cycle checkpoint
mitotic spindle assembly checkpoint
cell proliferation
viral process
cell division
regulation of chromosome segregation
spindle assembly checkpoint
cell cycle
chromosome segregation
embryo development
phosphorylation
mitotic spindle checkpoint
positive regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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