Invitrogen

CD141 Recombinant Mouse Monoclonal Antibody (JAA17)

Name: CD141 Recombinant Mouse Monoclonal Antibody (JAA17)
Brand: Invitrogen
SKU: 740179M

高级验证

查看其他43个Thrombomodulin (CD141)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)
高级验证 (1)

CD141 Antibody in Immunocytochemistry (ICC/IF)

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CD141 Antibody (740179M) in ICC/IF

Immunofluorescence analysis of CD141 was performed using CD141 Recombinant Mouse Monoclonal Antibody (JAA17) (Product # 740179M) on 70-80% confluent A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, and then blocked with 2% BSA for 1 hour at room temperature. The cells were stained with CD141 Recombinant Mouse Monoclonal antibody (JAA17) (Product # 740179M, 1:800) in 0.1% BSA, incubated at 4 degree Celsius overnight, followed by labelling with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alex... View More

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CD141 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested, owing to their inherent genetic constitution. CD141 Recombinant Mouse Monoclonal Antibody (JAA17) (Product # 740179M) specifically detects CD141 expression in A-431 cells (pink histogram) while low expression of CD141 is seen in Jurkat cells (blue histogram). {RE}

产品信息

740179M

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:800

流式细胞分析 (Flow)

0.125 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JAA17

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_3699440

产品详细信息

Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

Thrombomodulin (TM) is also known as Fetomodulin (FM), endothelial anticoagulant protein, or glycoprotein P112. Thrombomodulin contains six repeated domains homologous with epidermal growth factor (EGF) and an amino terminal domain homologous to lectin-like protein. Through its accelerated activation of protein C, synthesis of TM is one of several mechanisms important in maintaining thrombo-resistance and thus reducing clot formation on the surface of the endothelial cells. Immunohistochemical staining of formalin-fixed tissue for TM has been used for the study of a variety of vascular tumors, meningiomas and choriocarcinomas.

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