Invitrogen

CD160 Monoclonal Antibody (eBioCNX46-3 (CNX46-3)), Biotin, eBioscience™

Name: CD160 Monoclonal Antibody (eBioCNX46-3 (CNX46-3)), Biotin, eBioscience\u2122
Brand: Invitrogen
SKU: 13-1601-82

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2 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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CD160 Antibody (13-1601-82) in Flow

C57BL/6 splenocytes were stained with 0.25 µg of Anti-Mouse CD160 biotin followed by Streptavidin PE (Product # 12-4317-87), Anti-Mouse CD3e FITC (Product # 11-0031-82), and Anti-Mouse NK1-1 APC (Product # 17-5941-82). The histogram (right) demonstrates staining of CD160 on NK1-1+CD3. cells (red histogram) and NK1-1+CD3+ cells (black histogram), as gated in the dot plot (left).

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产品信息

13-1601-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioCNX46-3 (CNX46-3)

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10671017

产品详细信息

Description: CD160 is a glycosylphosphatidylinositol (GPI)-anchored Ig-like glycoprotein first identified on human lymphocytes with the monoclonal antibody BY55. In mice, CD160 is expressed on almost all (intestinal intraepithelial lymphocytes) iIELs, NKT cells, most TCRgammadelta T cells, few NK cells and a minor subset of CD8+ T cells. Murine CD160 has been shown to bind to a wide range of classical and non classical MHC class I molecules and regulate NK cell activation. In vitro, CD3 activation of murine CD8+ T cells increases the expression of CD160 and induces the release of soluble CD160 (sCD160). In human, CD160 mAb cross-linking triggers TNF alpha, IFN gamma and IL-6 cytokine production by peripheral blood NK cells and inhibits tube formation and induces apoptosis of endothelial cells. In mice, cross-linking of CD160 with the CNX46-3 antibody regulates NK cell activation both positively and negatively, depending on the stimulus.

Applications Reported: This eBioCNX46-3 (CNX46-3) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioCNX46-3 (CNX46-3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD160 is a receptor showing broad specificity for both classical and non-classical MHC class I molecules. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. It is involved in cell proliferation, cell surface receptor signaling pathways, cellular defense response, and regulation of immune response.

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