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CD4 Antibody (740028T) in Flow
Human PBMCs were stained with CD3 FITC (Product # 11-0036-42) and 0.5 µg of CD4 Recombinant Rat Monoclonal Antibody (4SM95) (Product # 740028T) (right dot plot) or left stained with 0.5 µg of Rat IgG1 (Isotype), followed by Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (Product # A78947). Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (Product # L34964). The flow cytometry data was acquired using Attune™ NxT Flow Cytometer (Pr... View More
产品信息
740028T
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rat
/ IgG1, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
4SM95
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
基因别名: CD4; CD4mut; L3T4; Ly-4
UniProt ID: (Human) P01730, (Mouse) P06332
Entrez Gene ID: (Human) 920, (Mouse) 12504
virus receptor activity
glycoprotein binding
receptor activity
transmembrane signaling receptor activity
extracellular matrix structural constituent
protein binding
zinc ion binding
coreceptor activity
immunoglobulin binding
enzyme binding
protein kinase binding
MHC class II protein binding
protein homodimerization activity
protein tyrosine kinase binding
cytokine production
immune system process
induction by virus of host cell-cell fusion
immune response
cell adhesion
cell surface receptor signaling pathway
T cell differentiation
maintenance of protein location in cell
T cell activation
T cell selection
protein palmitoleylation
positive regulation of protein kinase activity
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-negative bacterium
positive regulation of calcium-mediated signaling
regulation of T cell activation
positive regulation of T cell activation
adaptive immune response
signal transduction
enzyme linked receptor protein signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of calcium ion transport into cytosol
fusion of virus membrane with host plasma membrane
entry into host cell
T cell costimulation
response to estradiol
response to vitamin D
helper T cell enhancement of adaptive immune response
positive regulation of T cell proliferation
positive regulation of interleukin-2 biosynthetic process
regulation of defense response to virus by virus
T cell receptor signaling pathway
