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Identifyn

PARP1 SRM Recombinant Rabbit Monoclonal Antibody (BLR018M), Alexa Fluor™ 488, Identifyn™

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PARP1 SRM Recombinant Rabbit Monoclonal Antibody (BLR018M), Alexa Fluor™ 488, Identifyn™

Product Details

DC-000009

Applications
Tested Dilution
Publications

Western Blot (WB)

1:250-1:1,000
-

Immunocytochemistry (ICC/IF)

1:250-1,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

ExpiCHO

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR018M

Immunogen

Immunogen was a peptide representing the cleaved N-terminal of human Poly [ADP-ribose] polymerase 1 PARP1, a region between residue 210 and 265, referencing both Uniprot ID P09874 and Gene ID 142.
View immunogen

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

50 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 10% glycerol

Contains

0.02% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.

Recommended dilution for traditional microscopy:

Widefield - 1:250-1:1,000

Widefield SIM - 1:250-1:1,000

Confocal - 1:250-1:1,000

Recommended dilution for Super Resolution microscopy:

SIM (Structured Illumination Microscopy) - 1:1,000

Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:100-1:1,000

Detailed protocols can be found below under Documents & Downloads.

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase family, member 1; Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; Protein poly-ADP-ribosyltransferase PARP1; unnamed protein product

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Gene Aliases: ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PARP1; PARS; Poly-PARP; PPOL

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UniProt ID: (Human) P09874

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Entrez Gene ID: (Human) 142

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Function(s)
DNA binding chromatin binding damaged DNA binding RNA binding catalytic activity NAD+ ADP-ribosyltransferase activity protein binding enzyme activator activity zinc ion binding transferase activity transferase activity, transferring glycosyl groups nucleotidyltransferase activity enzyme binding protein kinase binding estrogen receptor binding nucleosome binding ubiquitin protein ligase binding identical protein binding protein homodimerization activity histone deacetylase binding metal ion binding NAD binding RNA polymerase II sequence-specific DNA binding transcription factor binding R-SMAD binding NAD DNA ADP-ribosyltransferase activity transcription regulator activator activity NAD+-protein-serine ADP-ribosyltransferase activity NAD+-protein-aspartate ADP-ribosyltransferase activity NAD+-protein-glutamate ADP-ribosyltransferase activity NAD+-protein-tyrosine ADP-ribosyltransferase activity NAD+-protein-histidine ADP-ribosyltransferase activity NAD+-histone H2BS6 serine ADP-ribosyltransferase activity NAD+-histone H3S10 serine ADP-ribosyltransferase activity NAD+-histone H2BE35 glutamate ADP-ribosyltransferase activity protein ADP-ribosylase activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter telomere maintenance double-strand break repair via homologous recombination immune system process DNA repair double-strand break repair transcription from RNA polymerase II promoter apoptotic process cellular response to DNA damage stimulus mitochondrion organization transforming growth factor beta receptor signaling pathway response to gamma radiation positive regulation of cardiac muscle hypertrophy carbohydrate biosynthetic process protein autoprocessing signal transduction involved in regulation of gene expression macrophage differentiation DNA ADP-ribosylation mitochondrial DNA metabolic process positive regulation of DNA-templated transcription, elongation cellular response to insulin stimulus regulation of protein localization positive regulation of intracellular estrogen receptor signaling pathway negative regulation of transcription elongation from RNA polymerase II promoter cellular response to oxidative stress cellular response to UV protein modification process positive regulation of I-kappaB kinase/NF-kappaB signaling mitochondrial DNA repair innate immune response regulation of circadian sleep/wake cycle, non-REM sleep negative regulation of innate immune response negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter decidualization positive regulation of mitochondrial depolarization positive regulation of SMAD protein import into nucleus positive regulation of necroptotic process protein poly-ADP-ribosylation protein auto-ADP-ribosylation protein localization to chromatin cellular response to zinc ion cellular response to transforming growth factor beta stimulus replication fork reversal DNA repair-dependent chromatin remodeling negative regulation of cGAS/STING signaling pathway transcription pausing by RNA polymerase II positive regulation of protein localization to nucleus cellular response to oxygen-containing compound regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway response to aldosterone negative regulation of adipose tissue development positive regulation of adipose tissue development negative regulation of telomere maintenance via telomere lengthening cellular response to beta-amyloid positive regulation of myofibroblast differentiation regulation of base-excision repair positive regulation of double-strand break repair via homologous recombination cellular response to nerve growth factor stimulus protein localization to site of double-strand break ATP generation from poly-ADP-D-ribose negative regulation of ATP biosynthetic process
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