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Identifyn
PARP1 Recombinant Rabbit Monoclonal Antibody (BLR018M), Alexa Fluor™ 405, Identifyn™
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FIGURE: 1 / 7
PARP1 Antibody (DC-000054) in ICC/IF
Identifyn Detailed protocols can be found below under Documents & Downloads. Confocal Microscopy: HeLa cells were grown on acid-etched 1.5H cover glass to ~50% confluency. DNA damage was induced via treatment with 2 µM Etoposide–spiked complete growth media for 24 hours, followed by -20°C methanol fixation and blocked with Thermo Fisher Fish Serum Blocker (Product # 37527X3). The primary antibody, Rabbit anti-Human PARP1 Recombinant Monoclonal - Identifyn SRM Alexa Fluor 405, IgG - (BLR018M), was incubated for 1 hour at room temperature at... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
DC-000054
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
ExpiCHO
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR018M
Immunogen
Immunogen was a synthetic peptide representing the cleaved N-terminal of human Poly [ADP-ribose] polymerase 1 PARP1, a region between residue 210 and 265, referencing both Uniprot ID P09874 and Gene ID 142.
Conjugate
Alexa Fluor™ 405
Alexa Fluor™ 405
Alexa Fluor™ 405
Excitation/Emission Max
401/422 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 10% glycerol
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan.
Recommended dilution for traditional microscopy:
Widefield - 1:250-1:1,000
Widefield SIM - 1:250-1:1,000
Confocal - 1:250-1:1,000
Recommended dilution for Super Resolution microscopy:
SIM (Structured Illumination Microscopy) 1:100-1:1,000
Detailed protocols can be found below under Documents & Downloads.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADP-ribosyltransferase diphtheria toxin-like 1; ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; Poly [ADP-ribose] polymerase 1; Poly[ADP-ribose] synthase 1; Protein poly-ADP-ribosyltransferase PARP1
Gene Aliases: ADPRT; PARP1; PPOL
Entrez Gene ID: (Human) 142
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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