Invitrogen

EOMES Monoclonal Antibody (Dan11mag), eFluor™ 450, eBioscience™

Name: EOMES Monoclonal Antibody (Dan11mag), eFluor\u2122 450, eBioscience\u2122
Brand: Invitrogen
SKU: 48-4875-82

52个已发表图片

46 篇参考文献

查看其他34个EOMES抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (52)

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EOMES Antibody (48-4875-82) in Flow

C57Bl/6 splenocytes were surface stained with Anti-Mouse NK1-1 APC (Product # 17-5941-82) followed by fixation and permeabilization using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells were intracellularly stained with 0.25 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.25 µg of Anti-Mouse EOMES eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

EOMES Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

EOMES Antibody in Immunocytochemistry (ICC/IF)

EOMES Antibody in Immunohistochemistry (Frozen) (IHC (F))

EOMES Antibody in Immunohistochemistry (IHC)

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产品信息

48-4875-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看13篇已发表文章 13篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看7篇已发表文章 7篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看25篇已发表文章 25篇已发表文章

产品规格

种属反应

Mouse

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

Dan11mag

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574062

产品详细信息

Description: This Dan11mag antibody recognizes Eomesodermin (EOMES), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This Dan11mag antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This Dan11mag antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

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