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Invitrogen
HuC/HuD Monoclonal Antibody (16A11)
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HuC/HuD Antibody (A-21271) in ICC/IF
Immunofluorescent analysis of HuC/D (green) and MAP2 (red) on rat primary cortical neurons cultured for 28 days in the B-27 Plus Neuronal Culture System (Product # A3653401). At day 28 the cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% triton x-100 for 30min, and blocked with 1% BSA for 30 min at room temperature. Cells were stained with anti-HuC/D antibody (Product # A-21271) at a dilution of 1:250, and anti-MAP2 (Product # PA5-17646) at a dilution of 1:250, in 1% BSA staining buffer, overnight at 4C, and th... View More
产品信息
A-21271
应用
建议稀释比
已发表文章
产品规格
种属反应
Avian,
Chicken,
Human,
Zebrafish
已发表种属
Amphibian,
Avian,
Bacteria,
C. elegans,
Cat,
Chicken,
Chimpanzee,
Fish,
Guinea pig,
Horse,
Human,
Lizard,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Reptile,
Rhesus monkey,
Rodent,
Shark,
Sheep,
Xenopus,
Zebrafish
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
16A11
抗原
Human HuC/HuD neuronal protein.
偶联物
形式
Lyophilized
纯化类型
purified
保存液
HEPES buffered saline
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle.
Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20°C or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4°C with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20°C, avoidig freeze/thaw cycles.
For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes.
A-21271 was successfully used to detect HuC/HuD in neurons differentiated from H9 ESC derived NSCs.
靶标信息
Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C); ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); ELAV-like protein 3; ELAV-like protein 4; elav-related C; elav-related D; elavC; embryonic lethal, abnormal vision-related C; embryonic lethal, abnormal vision-related D; etID309927.3; Hu antigen C; Hu antigen D; Hu-antigen C; Hu-antigen D; HuC; HuD; Paraneoplastic cerebellar degeneration-associated antigen; Paraneoplastic encephalomyelitis antigen HuD; Paraneoplastic limbic encephalitis antigen 21; Protein elrD; RNA-binding protein HuD
基因别名: ELAVL3; ELAVL4; elrc; elrd; HUC; HUCL; HUD; id:ibd1248; PLE21; PNEM; wu:fb77b03; wu:fc24h01; zHuC
UniProt ID: (Human) Q14576, (Human) P26378, (Zebrafish) A0A0R4IEW8
Entrez Gene ID: (Human) 1995, (Chicken) 395634, (Human) 1996, (Zebrafish) 30732, (Zebrafish) 30737
