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Invitrogen
TRA-1-81 Monoclonal Antibody (TRA-1-81)
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TRA-1-81 Antibody (MA1-024) in ICC/IF
Immunofluorescent analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product # MA1-024) shows staining on the cell surface of human H9 human embryonic stem cells, indicating pluripotency. TRA-1-81 staining (green) and an overlay image of TRA-1-81 with DAPI (blue) is shown. H9 human embryonic stem cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing TRA-1-81 (Product # MA1-024) at a dilution of 1:20 overnight at 4 °C, washed with... View More
产品信息
MA1-024
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Bovine,
C. elegans,
Human
宿主/亚型
Mouse
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
TRA-1-81
抗原
human embryonal carcinoma cell line 2102Ep
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography - MBP
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
