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Invitrogen
TRA-1-81 Monoclonal Antibody (TRA-1-81), DyLight™ 550
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TRA-1-81 Antibody (MA1-024-D550) in ICC/IF
Immunofluorescent analysis of TRA-1-81 (red) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 550-conjugated TRA-1-81 monoclonal antibody (Product # MA1-024-D550) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. Nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magni... View More
产品信息
MA1-024-D550
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
TRA-1-81
抗原
human embryonal carcinoma cell line 2102Ep
偶联物
激发/发射光谱
556/570 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography - MBP
保存液
PBS with proprietary stabilizer
内含物
0.02% sodium azide
保存条件
4°C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-024-D550 has been successfully used in ICC/IF applications on human samples.
靶标信息
TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
