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Invitrogen
Bub1 Monoclonal Antibody (14H5)
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Product Details
MA1-5755
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
14H5
Immunogen
Fusion protein, corresponding to amino acids 281-419 of Human Bub 1
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Amount
110 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-5755 detects BUB1 in Human samples and is predicted to detect BUB1 in Rat, Xenopus laevis, and Kangaroo.
MA1-5755 has been successfully used in immunofluorescence and immunoprecipitation procedures.
Target Information
The BUB1 gene encodes a serine/threonine-protein kinase that is integral to the spindle assembly checkpoint, a crucial mechanism ensuring proper chromosome alignment and segregation during mitosis. BUB1 is involved in detecting and correcting errors in kinetochore-microtubule attachments, which is vital for maintaining genomic stability. It acts by delaying the onset of anaphase until all chromosomes are correctly attached to the spindle apparatus. BUB1 is recruited to kinetochores in early mitosis, where it phosphorylates several substrates to activate other checkpoint proteins, such as BUBR1 and MAD2, thereby preventing premature progression through mitosis. Mutations or aberrations in the expression levels of BUB1 are often associated with aneuploidy and chromosomal instability, contributing to cancer progression. BUB1 is thus considered a potential target for cancer therapeutics, aiming to exploit its role in the mitotic checkpoint to selectively eliminate tumor cells through checkpoint bypass or mitotic arrest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1A; budding uninhibited by benzimidazoles 1 homolog; hBUB1; huBUB1; HuBub1; putative serine/threonine-protein kinase; mBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; protein kinase; putative serine/threonine-protein kinase; unnamed protein product
Gene Aliases: AL022991; BUB1; BUB1A; BUB1L; C80208; D2Xrf87; hBUB1; MCPH30
UniProt ID: (Human) O43683, (Mouse) O08901
Entrez Gene ID: (Human) 699, (Mouse) 12235
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein serine kinase activity
histone H2A kinase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
chromatin remodeling
apoptotic process
chromosome segregation
regulation of sister chromatid cohesion
mitotic spindle assembly checkpoint
intracellular signal transduction
cell division
meiotic sister chromatid cohesion, centromeric
regulation of chromosome segregation
positive regulation of maintenance of mitotic sister chromatid cohesion, centromeric
protein phosphorylation
cell cycle
mitotic nuclear division
embryo development
phosphorylation
spindle assembly checkpoint
mitotic spindle checkpoint
positive regulation of intrinsic apoptotic signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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