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Invitrogen
Mouse IgM Monoclonal Antibody (II/41), eFluor™ 570, eBioscience™
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图: 1 / 12
Mouse IgM Antibody (41-5790-82) in ICC/IF
Immunocytochemistry on fixed, differentiated OLN93 cells using 10 µg/mL Mouse IgM Isotype Control Purified (left) or 10 µg/mL Anti-Oligodendrocyte Marker O1 Purified (right) followed by 10 µg/mL Anti-Mouse IgM eFluor® 570. Nuclei are counterstained with DAPI.
产品信息
41-5790-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
II/41
偶联物
激发/发射光谱
556/569 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.
Applications Reported: This II/41 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, and immunocytochemistry.
Applications Tested: This II/41 antibody has been tested by immunocytochemistry on fixed, differentiated OLN93 cells with O1 (Anti-Oligodendrocyte Marker) primary antibody, and by immunohistochemistry (IHC-P) on FFPE human tonsil with HI98 (Anti-CD15) as the primary antibody. This II/41 antibody can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable.
Excitation: 555 nm; Emission: 570 nm
靶标信息
Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名: AI326478; Igh-6; Igh-M; Igh6; Igm; muH
Entrez Gene ID: (Mouse) 16019
