Invitrogen

Myosin 4 Monoclonal Antibody (MF20), eFluor™ 660, eBioscience™

Name: Myosin 4 Monoclonal Antibody (MF20), eFluor\u2122 660, eBioscience\u2122
Brand: Invitrogen
SKU: 50-6503-82

高级验证

13个已发表图片

23 篇参考文献

查看其他9个Myosin 4抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Myosin 4 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 18

Myosin 4 Antibody (50-6503-82) in ICC/IF

Immunofluorescence analysis of MYH4 was performed using 70% confluent log phase C2C12 cells and C2C12 cells differentiated to Myotubes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Myosin 4 Monoclonal Antibody (MF20), eFluor 660, eBioscience™ (Product # 50-6503-82) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L)... View More

Myosin 4 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Myosin 4 Antibody in Immunohistochemistry (IHC)

Myosin 4 Antibody in Flow Cytometry (Flow)

Product Image Gallery

产品信息

50-6503-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

查看12篇已发表文章 12篇已发表文章

流式细胞分析 (Flow)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Bovine, Dog, Chicken, Chimpanzee, Cat, Guinea pig, Human, Mouse, Non-human primate, Rabbit, Rat

已发表种属

C. elegans, Human, Mouse, Pig

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

MF20

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574267

产品详细信息

Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis.

The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog.

Applications Reported: This MF20 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of frozen tissue sections.

Applications Tested: This MF20 antibody has been tested immunocytochemistry on fixed and permeabilized C2C12 cells at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Target protein includes a fast myosin isoform expressed in skeletal muscle and has function involved in Muscle contraction.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 2B myosin heavy chain; fast myosin heavy chain 2B; myh4; MyHC IIb isoform; MyHC-2b; MyHC-IIb; Myosin heavy chain 2b; myosin heavy chain 2X; Myosin heavy chain 4; Myosin heavy chain IIb; myosin heavy chain type IIb; Myosin heavy chain, skeletal muscle; Myosin heavy chain, skeletal muscle, fetal; Myosin heavy chain, skeletal muscle, juvenile; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 4, skeletal muscle; Myosin-4; unnamed protein product

查看更多收起

基因别名: AI506973; MHC; MHC2B; Minimsc; Minmus; MM; MYH-2B; MYH1; MYH2B; MYH4; MyHC-2B; MyHC-IIb; Myhsf