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Invitrogen
NPC1 Polyclonal Antibody
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FIGURE: 1 / 1
NPC1 Antibody (PA5-79754) in WB
Western blot analysis of NPC1 in Lane 1: rat pancreas tissue lysate, Lane 2: mouse NIH3T3 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with NPC1 polyclonal antibody (Product # PA5-79754) at a dilution of 0.5 µg/mL (overnight at 4°C), followed b... View More
Product Details
PA5-79754
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human Niemann Pick C1 recombinant protein (Position: A1022-F1278).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat pancreas tissue, mouse NIH3T3 whole cell.
Target Information
Mutations in the Niemann-Pick disease type C1 (NPC1) gene result in a fatal progressive neurodegenerative disorder characterized by an abnormal sequestration of lipids including cholesterol and glycosphingolipids. The NPC1 protein is a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. NPC1 transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolyzed and released as free cholesterol. NPC1, in addition to FTO, MC4R, and PTER has recently been shown to be a new risk loci for early-onset and morbid adult obesity in European populations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ98532; Niemann Pick type C1; Niemann-Pick C1 protein; NPC intracellular cholesterol transporter 1; sphingomyelinosis; unnamed protein product
Gene Aliases: A430089E03Rik; C85354; Cdig2; D18Ertd139e; D18Ertd723e; lcsd; nmf164; NPC; NPC1; POGZ; SLC65A1; spm
UniProt ID: (Human) O15118, (Mouse) O35604
Entrez Gene ID: (Human) 4864, (Mouse) 18145, (Rat) 266732
protein glycosylation
lipid metabolic process
endocytosis
autophagy
adult walking behavior
steroid metabolic process
cholesterol metabolic process
negative regulation of macroautophagy
cholesterol transport
membrane raft organization
cholesterol efflux
response to drug
cholesterol homeostasis
response to cadmium ion
viral entry into host cell
negative regulation of cell death
cellular response to steroid hormone stimulus
cellular response to low-density lipoprotein particle stimulus
establishment of protein localization to membrane
positive regulation of cholesterol homeostasis
signal transduction
liver development
lipid transport
lysosomal transport
bile acid metabolic process
response to xenobiotic stimulus
gene expression
cholesterol storage
programmed cell death
sterol transport
sterol metabolic process
macroautophagy
neurogenesis
intestinal cholesterol absorption
intracellular lipid transport
intracellular cholesterol transport
establishment of localization in cell
negative regulation of epithelial cell apoptotic process
negative regulation of TORC1 signaling
cyclodextrin metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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