Invitrogen

PERK Polyclonal Antibody

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View all (27) PERK antibodies

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PERK Polyclonal Antibody

Product Details

PA5-120620

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-

Affinity Purification (AP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

C. elegans

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 867-1116 of human PERK (NP_004827.4).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.99 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2914192

Product Specific Information

Positive test controls include: Mouse thymus. The target is usually found in the following locations: Endoplasmic reticulum membrane, Single-pass type I membrane protein.

Immunogen sequence: LTKNTTEKLQ PSSPKVYLYI QMQLCRKENL KDWMNGRCTI EERERSVCLH IFLQIAEAVE FLHSKGLMHR DLKPSNIFFT MDDVVKVGDF GLVTAMDQDE EEQTVLTPMP AYARHTGQVG TKLYMSPEQI HGNSYSHKVD IFSLGLILFE LLYPFSTQME RVRTLTDVRN LKFPPLFTQK YPCEYVMVQD MLSPSPMERP EAINIIENAV FEDLDFPGKT VLRQRSRSLS SSGTKHSRQS NNSHSPLPSN

Target Information

EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 2.7.11.1; Eukaryotic translation initiation factor 2-alpha kinase 3; HsPEK; OTTHUMP00000207187; OTTHUMP00000207188; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase; Protein tyrosine kinase EIF2AK3

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Gene Aliases: AI427929; EIF2AK3; PEK; PERK; WRS

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UniProt ID: (Human) Q9NZJ5, (Rat) Q9Z1Z1, (Mouse) Q9Z2B5

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Entrez Gene ID: (Human) 9451, (Rat) 29702, (Mouse) 13666

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Function(s)
protein kinase activity protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity protein binding ATP binding enzyme binding protein phosphatase binding identical protein binding protein homodimerization activity Hsp90 protein binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups eukaryotic initiation factor eIF2 binding non-receptor serine/threonine protein kinase
Process(es)
skeletal system development ossification angiogenesis chondrocyte development protein phosphorylation activation of cysteine-type endopeptidase activity involved in apoptotic process ER overload response endoplasmic reticulum organization positive regulation of vascular endothelial growth factor production positive regulation of gene expression regulation of translational initiation by eIF2 alpha phosphorylation negative regulation of translation peptidyl-serine phosphorylation calcium-mediated signaling insulin secretion bone mineralization endoplasmic reticulum unfolded protein response endocrine pancreas development negative regulation of myelination negative regulation of translational initiation in response to stress cellular response to amino acid starvation response to endoplasmic reticulum stress eiF2alpha phosphorylation in response to endoplasmic reticulum stress PERK-mediated unfolded protein response cellular response to glucose starvation positive regulation of transcription from RNA polymerase I promoter protein autophosphorylation insulin-like growth factor receptor signaling pathway protein homooligomerization regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation cellular response to cold positive regulation of protein localization to nucleus regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway response to manganese-induced endoplasmic reticulum stress translation lactation positive regulation of signal transduction negative regulation of gene expression regulation of fatty acid metabolic process pancreas development negative regulation of translation in response to stress positive regulation of protein binding SREBP signaling pathway negative regulation of apoptotic process fat cell differentiation intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway regulation of translation response to unfolded protein phosphorylation negative regulation of translation in response to endoplasmic reticulum stress positive regulation of glutathione biosynthetic process positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
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