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Invitrogen
Phospho-4EBP1 (Thr36, Thr45) Recombinant Mouse Monoclonal Antibody (V3NTY24), Alexa Fluor™ Plus 647
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Phospho-4EBP1 (Thr36, Thr45) Antibody (740048MP647) in WB
Western blot was performed using Phospho-4EBP1 (Thr36, Thr45) Recombinant Mouse Monoclonal Antibody (V3NTY24), Alexa Fluor™ Plus 647 (Product # 740048MP647) and a ~17 kDa band corresponding to Phospho-4EBP1 was observed in NIH/3T3 serum starved for 24hrs and NIH/3T3 serum starved for 24hrs followed by treatment with recombinant mouse PDGF BB, 100 ng/mL for 30 min and pervanadate, 1 mM for 30 min. Whole cell... View More
产品信息
740048MP647
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2b, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
V3NTY24
偶联物
激发/发射光谱
658/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
靶标信息
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E, which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 4E-BP1; eIF4E-binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC431; MGC4316; P/OKCL.6; PHAS-1; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1
基因别名: 4E-BP1; 4EBP1; BP-1; EIF4EBP1; PHAS-I
UniProt ID: (Human) Q13541
Entrez Gene ID: (Human) 1978
G1/S transition of mitotic cell cycle
IRES-dependent translational initiation
response to ischemia
insulin receptor signaling pathway
lung development
negative regulation of protein complex assembly
TOR signaling
response to ethanol
positive regulation of mitotic cell cycle
negative regulation of translational initiation
cellular response to hypoxia
