Invitrogen

Phospho-Syk (Tyr348) Monoclonal Antibody (moch1ct), APC, eBioscience™

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产品信息

17-9014-42

应用
建议稀释比
已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test
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产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

推荐的同型对照

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

moch1ct

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱 spectra

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573276

产品详细信息

Description: This moch1ct monoclonal antibody recognizes human and mouse spleen tyrosine kinase (also known as SYK) when phosphorylated on tyrosine 348 (Y348). SYK is the founding member of the SYK family of kinases that also includes ZAP-70 (zeta-associated protein of 70 kD) and is expressed in hematopoietic cells, including B lymphocytes, immature (CD4, CD8 double-negative and double-positive) thymocytes, and myeloid cells, epithelial cell lines, and normal breast tissue. SYK is critical for B cell receptor (BCR) signaling and B cell development. Autophosphorylation of at Y348 is necessary for SYK to become fully catalytically active and creates a docking site for the SH2 domains of Vav, Grb2, p85 subunit of PI3 kinase, and PLC gamma.

Specificity of this moch1ct clone was confirmed by ELISA, flow cytometry, and western blotting.

Applications Reported:This moch1ct antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This moch1ct antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated mouse splenocytes. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the BestProtocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols® Section under the Resources tab online.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72-Syk; SP1; Spleen tyrosine kinase; syk signaling; tyrosine-kinase syk; Tyrosine-protein kinase SYK

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基因别名: p72-Syk; ptk72; SYK; Sykb

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UniProt ID: (Human) P43405, (Mouse) P48025

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Entrez Gene ID: (Human) 6850, (Mouse) 20963

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功能
protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity integrin binding protein binding ATP binding protein kinase binding nucleotide binding receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein domain specific binding kinase modulator
参与通路
angiogenesis lymph vessel development positive regulation of receptor internalization stimulatory C-type lectin receptor signaling pathway adaptive immune response macrophage activation involved in immune response neutrophil activation involved in immune response leukocyte activation involved in immune response serotonin secretion by platelet protein phosphorylation inflammatory response leukocyte cell-cell adhesion transmembrane receptor protein tyrosine kinase signaling pathway integrin-mediated signaling pathway activation of JUN kinase activity cell proliferation organ morphogenesis regulation of platelet activation regulation of tumor necrosis factor-mediated signaling pathway viral process peptidyl-serine phosphorylation leukotriene biosynthetic process platelet activation neutrophil chemotaxis receptor internalization positive regulation of type I interferon production regulation of superoxide anion generation positive regulation of cell adhesion mediated by integrin peptidyl-tyrosine autophosphorylation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis defense response to bacterium transcription factor import into nucleus positive regulation of mast cell degranulation regulation of neutrophil degranulation beta selection innate immune response positive regulation of interleukin-3 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of bone resorption positive regulation of alpha-beta T cell differentiation positive regulation of alpha-beta T cell proliferation blood vessel morphogenesis positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation regulation of phagocytosis positive regulation of calcium-mediated signaling B cell receptor signaling pathway regulation of sequence-specific DNA binding transcription factor activity regulation of ERK1 and ERK2 cascade cellular response to molecule of fungal origin cellular response to low-density lipoprotein particle stimulus regulation of arachidonic acid secretion regulation of platelet aggregation activation of MAPK activity serotonin secretion immune system process cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway G-protein coupled receptor signaling pathway phosphorylation cell migration peptidyl-tyrosine phosphorylation signal transduction by protein phosphorylation cell differentiation intracellular signal transduction protein autophosphorylation regulation of immune response
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