Invitrogen

RAGE Polyclonal Antibody

Name: RAGE Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-075

高级验证

9个已发表图片

18 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

RAGE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RAGE Antibody (PA1-075) in IHC (P)

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal... View More

RAGE Antibody in Immunohistochemistry (IHC)

RAGE Antibody in Western Blot, Neutralization (WB, Neu)

Product Image Gallery

RAGE Antibody — Figure 1. High-mobility group box1 (HMGB1) upregulated MUC5AC and MUC5B protein expression, and gene regulation was blocked by a receptor for advanced glycation end products (RAGE) inhibitor. Normal human bronchial epithelial (NHBE) cells were cultured at the air-liquid interface, treated with HMGB1 (10 or 100 ng/mL) for 24 or 48 hours, and analyzed for relative MUC5AC (A) and MUC5B (B) protein levels in the apical media by microtiter plate assay ( see the data supplement). Involvement of the RAGE receptor in HMGB1-induced MUC5AC (C) and MUC5B (D) mRNA expression was tested by pretreatment with a RAGE inhibitor, FPS-ZMI (500 nM), for 1 hour, followed by administration of HMGB1 (10 ng/mL, 24 h). Expression of MUC5AC and MUC5B mRNA was determined by qRT-PCR as described in the data supplement. HMGB1 (10 or 100 ng/mL; 24-48 h) increased AGER (RAGE) mRNA expression (E) and RAGE protein levels as shown by densitometric analysis (F) of Western blots in well-differentiated, primary NHBE cells. A representative Western blot for RAGE is shown in G. RAGE mRNA was determined by qRT-PCR, and RAGE protein levels were compared with vehicle control-treated cells by Western blot, as described in the data supplement. Data are expressed as mean +- SEM. P < 0.05 was considered statistically significant (* P < 0.05, ** P < 0.01, and *** P < 0.001 comparisons between conditions are marked by lines). Two to five independent experiments with six or more replic {TM}

Immunogen image

Immunogen Image RAGE Polyclonal Antibody

产品信息

PA1-075

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:10-1:100

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免疫组化（冰冻） (IHC (F))

1-2 µg/mL

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中和实验 (Neu)

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产品规格

种属反应

Mouse, Rat

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2269243

产品详细信息

PA1-075 detects receptor for advanced glycation endproducts (RAGE) from mouse and rat samples.

PA1-075 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects two bands in the 45 kDa range representing the RAGE protein pre and post-glycosylation in mouse lung extract. This antibody also detects an ~25 kDa protein that is believed to be proteolytic degradation product. Immunohistochemical staining of RAGE in transgenic mouse retina results in staining of the retinal pigmented epithelium and photo receptor cell layers.

The PA1-075 immunogen is a synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE. This sequence is 100% conserved in mouse and 70% conserved in humans. This peptide (Cat. # PEP-194) is available for use in neutralization and control experiments.

靶标信息

The Receptor for Advanced Glycation End-products (RAGE) is a gene located on human chromosome 6p21.3, encoding a transmembrane receptor belonging to the immunoglobulin superfamily. RAGE is expressed in various tissues, with significant levels in the lungs, and plays a crucial role in cellular signaling and inflammation. As a receptor, RAGE binds multiple ligands, including advanced glycation end-products (AGEs), amyloid-β peptide, high mobility group box 1 (HMGB1), and S100/calgranulin proteins, facilitating diverse pathological processes like inflammation, cancer progression, and neurodegeneration. The interaction between RAGE and its ligands triggers intracellular signaling pathways such as NF-?B activation, leading to inflammatory responses and oxidative stress. In the context of chronic diseases like diabetes, Alzheimer's, and cardiovascular diseases, RAGE is a critical mediator, linking metabolic disturbance to cellular dysfunction. Therapeutic targeting of RAGE signaling is under investigation, aiming to mitigate its contribution to inflammatory and degenerative diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: advanced glycation end-products receptor; Advanced glycosylation end product-specific receptor; advanced glycosylation end product-specific receptor variant 2; advanced glycosylation end product-specific receptor variant 3; advanced glycosylation end product-specific receptor variant 4; advanced glycosylation end product-specific receptor variant 5; RAGE-4 ORF3; receptor for advanced glycation end-products variant 20; Receptor for advanced glycosylation end products; sRAGE

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基因别名: Ager; RAGE

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UniProt ID: (Mouse) Q62151

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Entrez Gene ID: (Rat) 81722, (Mouse) 11596

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功能

protein binding identical protein binding S100 protein binding advanced glycation end-product receptor activity high mobility group box 1 binding cell adhesion molecule immunoglobulin superfamily cell adhesion molecule

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