Invitrogen

RAP1GAP Polyclonal Antibody

Name: RAP1GAP Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-87968

查看其他15个RAP1GAP抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

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RAP1GAP Antibody (PA5-87968) in WB

Western blot analysis of RAP1GAP in extracts of various cell lines. Samples were incubated with RAP1GAP Polyclonal antibody (Product # PA5-87968) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

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Immunogen image

Immunogen Image RAP1GAP Polyclonal Antibody

产品信息

PA5-87968

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 508-727 of human Rap1GAP (NP_0011391301)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.56 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2804549

产品详细信息

Immunogen sequence: AMGLSNKKPN TVSTSHSGSF APNNPDLAKA AGISLIVPGK SPTRKKSGPF GSRRSSAIGI ENIQEVQEKR ESPPAGQKTP DSGHVSQEPK SENSSTQSSP EMPTTKNRAE TAAQRAEALK DFSRSSSSAS SFASVVEETE GVDGEDTGLE SVSSSGTPHK RDSFIYSTWL EDSVSTTSGG SSPGPSRSPH PDAGKLGDPA CPEIKIQLEA SEQHMPQLGC; Positive Samples: U-87MG, 293T, SGC-7901, LO2; Cellular Location: Golgi apparatus membrane, Peripheral membrane protein

靶标信息

Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated G alpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。