Invitrogen

RAP1GAP Recombinant Rabbit Monoclonal Antibody (8V9I6)

Name: RAP1GAP Recombinant Rabbit Monoclonal Antibody (8V9I6)
Brand: Invitrogen
SKU: MA5-42831

查看其他15个RAP1GAP抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

RAP1GAP Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

RAP1GAP Antibody (MA5-42831) in ICC/IF

Immunofluorescence analysis of RAP1GAP in NIH-3T3 cells. Samples were incubated with RAP1GAP Monoclonal antibody (Product # MA5-42831) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Immunogen image

Immunogen Image RAP1GAP Recombinant Rabbit Monoclonal Antibody (8V9I6)

产品信息

MA5-42831

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

8V9I6

抗原

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rap1GAP (P47736).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.66 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2911972

产品详细信息

Positive test controls include: BxPC-3, U-87MG, Mouse brain, Rat brain. The target is usually found in the following locations: Golgi apparatus membrane, Peripheral membrane protein.

Immunogen sequence: MIEKMQGSRM DEQRCSFPPP LKTEEDYIPY PSVHEVLGRE GPFPLILLPQ FGGYWIEGTN HEITSIPETE PLQSPTTKVK LECNPTARIY RKHFLGKEHF

靶标信息

Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated G alpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.

仅用于科研。不用于诊断过程。未经明确授权不得转售。